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52486
Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B
Synonim(y):
TCEP-Agarose, Tris(2-carboxyethyl)phosphine-Agarose
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 759,00 zł | |
| 5 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2950,00 zł |
Informacje o tej pozycji
NACRES:
NA.25
UNSPSC Code:
12352204
759,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Level
storage temp.
2-8°C
Application
Tris(2-carboxyethyl)phosphine, immobilized on agarose CL-4B may be used in preliminary off-line experiments to study its ability to cleave disulfide bonds by both under flow conditions using sequential injection analysis (SI). It may also be used as a reducing agent in bio-tests for disrupting the disulfide bonds within and between the proteins.
Biochem/physiol Actions
Tris(2-carboxyethyl)phosphine also referred to as TCEP reduces the disulfides by the usual mechanism of reduction of disulfides by phosphines in water. Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B,Trialkylphosphines (TCEP) are highly effective agents for reducing disulfide bonds in proteins, peptides and other disulfide bondcontaining molecules and are relatively non-reactive toward other functional groups. The trialkylphosphine TCEP was first described by Levison et al. as an odorless and efficient reductant of alkyl disulfides over a wide pH range. TCEP is stable in aqueous solutions and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and β-mercaptoethanol (BME, 2-ME). TCEP does not interfere with commonly used sulfhydryl-reactive reagents (e.g., maleimide crosslinkers). Nevertheless, many protocols require recovery of the reduced sample separate from the reducing agent. Our Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B eliminates the need to use laborious and troublesome gel filtration methods to separate the reduced sample from the reducing agent. Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats. Examples are given for batch, spin cup column and gravity-flow column procedures. For small-scale reductions, the most complete sample recovery is made using the spin-cup column procedure (see Related Thermo Scientific Products Section).
Analysis Note
Bio-tests
Gel has an effective TCEP concentration ≥8 μmol/mL gel
Gel has an effective TCEP concentration ≥8 μmol/mL gel
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Ta pozycja | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Powiązane treści
Preparation of Avidin Conjugates
Haugland, R.P. and Blalgat, M.K et al.
Methods in Molecular Biology, 189-190 (1998)
On-line cleavage of disulfide bonds by soluble and immobilized tris-(2-carboxyethyl)phosphine using sequential injection analysis
Tzanavaras PD, et al.
Talanta (2012)
J C Han et al.
Analytical biochemistry, 220(1), 5-10 (1994-07-01)
The concentration of tris(2-carboxyethyl)phosphine (TCEP) can be conveniently determined by measuring the amount of 2-nitro-5-thiobenzoate (NTB) formed after reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). This method utilizes the fact that TCEP reduces DTNB rapidly and stoichiometrically to generate two equivalents of
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 52486-1ML | 04061837255458 |
| 52486-5ML | 04061837255465 |