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Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR RED antibody produced in goat

for STED application

Synonim(y):

Abberior® STAR RED-Anti-Rabbit IgG antibody produced in goat

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

pochodzenie biologiczne

goat

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Formularz

buffered aqueous solution

reaktywność gatunkowa

rabbit

stężenie

~1 mg/mL

fluorescencja

λex 638 nm; λem 655 nm in PBS, pH 7.4

temp. przechowywania

−20°C

Opis ogólny

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto 647N, Alexa Fluor 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

Cechy i korzyści

  • Unmatched, background free STED imaging cont
  • Verified in Abberior Instruments and Leica STED microscopes

Przydatność

Designed and tested for fluorescent super-resolution microscopy

Komentarz do analizy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Informacje prawne

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Kod klasy składowania

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Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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