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EX-CELL® 420 Serum-Free Medium for Insect Cells

EX-CELL® 420 Serum-Free Medium for Insect Cells

with L-glutamine, sterile-filtered, suitable for insect cell culture

Produkcja farmaceutyczna

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500 ML
315,00 zł
1000 ML
524,00 zł

315,00 zł

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500 ML
315,00 zł
1000 ML
524,00 zł

About This Item

Numer MDL:
MFCD01634638
Kod UNSPSC:
12352207
NACRES:
NA.75

315,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

opis

for research or for further manufacturing use

Poziom jakości

500

sterylność

sterile-filtered

linia produktu

EX-CELL®

Formularz

liquid

metody

cell culture | insect: suitable

komponenty

HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Zastosowanie

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

Informacje prawne

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000309423
0000316380
0000309423
0000308786
0000309399

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)
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  1. USA
    • Review 1
    • Votes 0
    3 out of 5 stars.

    Great if you can get it in the size you need it...

    We have used this great product for years, even prior to the acquisition by Sigma. Would really appreciate the operations improving to have the 10L bag size available, even prior to the pandemic this has been very poorly handled. Lack of bags to fill, failing QC...so we adjust by buying the smaller size at much higher cost and risk failed runs by having to manipulate filling our own bio-bags with numerous 1L size bottles.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review and we apologize for the inconvenience. The 1L and 500ml are our only regularly stocked package sizes at this time, but will pass on your feedback to our Product Managers for future package size considerations. While our BioEaze bag and rigging products have been discontinued there are other product options available, such as our Mobius system. This format is offered on a custom basis only. We encourage you to contact your Account Manager for a quote and lead time. We hope to hear from you soon.

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