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Merck

T1438

Millipore

Todd Hewitt broth

suitable for microbiology, NutriSelect® Plus

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About This Item

Kod UNSPSC:
41171607
NACRES:
NA.85

sterylność

non-sterile

Poziom jakości

Postać

powder

okres trwałości

limited shelf life, expiry date on the label

producent / nazwa handlowa

NutriSelect® Plus

metody

microbiological culture: suitable

pH

7.8±0.2 (25 °C)

Zastosowanie

clinical testing
food and beverages

microbiology

temp. przechowywania

2-25°C

przydatność

nonselective for Streptococcus spp.

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Opis ogólny

Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.

Zastosowanie

Recommended for the cultivation of group A hemolytic Streptococci used for serological studies.
Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.

Komponenty

Components (g/L):
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50

Uwaga dotycząca przygotowania

Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Przypis

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informacje prawne

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, type N95 (US)


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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Todd, E.W., et al.
The Journal of Pathology and Bacteriology, 35, 1-97 (1932)
Moody, M.D., et al.
American Journal of Public Health, 53, 1083-1083 (1963)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Observation of Pneumococcal Phase Variation in Colony Morphology
Li J, et al.
Bio-protocol, 7(15) (2017)
Handbook of Microbiological Media Third Edition (2004)

Produkty

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

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