For staining dinoflagellates using Neutral Red powder, it is recommended to use the same basic formulation as the Neutral Red solution offered as catalog number N2889, which is stated to be 3.3 g/L dissolved in a DPBS. It should be possible to dissolve in any suitable cell culture media that does not contain phenol red in the formulation. Neutral Red is a vital stain, so the dye needs to be dissolved into an isotonic solution and should not be dissolved in water.
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861251
Neutral Red
certified by the BSC
Synonim(y):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
25 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 649,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C15H17ClN4
Numer CAS:
Masa cząsteczkowa:
288.78
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
209-035-8
MDL number:
Beilstein/REAXYS Number:
3918740
Colour Index Number:
50040
649,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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certified by the BSC
Quality Segment
form
powder
composition
Dye content, 50-60%
technique(s)
titration: suitable
pH
6.8-8.0, red to yellow
mp
290 °C (dec.) (lit.)
solubility
water: 1 mg/mL
λmax
540 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
InChI
1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChI key
PGSADBUBUOPOJS-UHFFFAOYSA-N
Application
Certified for use in the method of Doan & Ralph for the supravital staining of living blood cells.
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Neutral red has been used as a staining agent for micro- and meso-plankton. It has been used as a staining agent for nematocytes.
Biochem/physiol Actions
Neutral red is useful as an indicator for preparing neutral red paper, and as a biological stain. Neutral dye has low toxicity and is a vital dye. It can also be used as a pH indicator. It is used for cell viability assay. The dye accumulates in the lysosomes of living healthy cells, but not in damaged cells.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - |
| form powder | form powder or crystals | form powder | form solid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility water: 1 mg/mL | solubility water: 0.01 g/10 mL | solubility water: 10 mg/mL, dark red | solubility 50 g/L |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| composition Dye content, 50-60% | composition - | composition Dye content, ≥90% | composition Dye, ≥90% spectrophotometric assay |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How to prepare Neutral Red 861251 for staining dinoflagellates?
1 answer-
Helpful?
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What percentage is this neutral red solution?
1 answer-
The dye content specification for this product is 50 - 60% Neutral Red. The exact percentage is lot-specific and will be reported on the Certificate of Analysis. See the link below for a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIAL/861251/11714LEHelpful?
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