Wszystkie zdjęcia(1)
Kluczowe dokumenty
11681460001
Roche
Roztwór podstawowy INT/BCIP
solution, pkg of 3 mL
Synonim(y):
BCIP, Fosforan 5-bromo-4-chloro-3-indoilowy
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352204
Polecane produkty
Formularz
solution
masa cząsteczkowa
(INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6)
opakowanie
pkg of 3 mL
producent / nazwa handlowa
Roche
temp. przechowywania
2-8°C
Opis ogólny
BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product.[1] INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water.
Zastosowanie
Charakterystyka techniczna
Formulas: INT: C19H13CIN5O2; BCIP: C8H6NO4BrCIP x C7H9N
Zasada
BCIP is the AP-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT is the oxidant producing a red dye.
Postać fizyczna
Solution of 33 mg/ml INT (2-[4-iodophenyl]-3-[4-nitrophenyl]-5-phenyltetrazolium chloride) and 33 mg/ml BCIP (5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in DMSO)
Uwaga dotycząca przygotowania
Working solution: Preparation of 10 ml Staining Solution
Bring the stock solution to 15 to 25 °C until all components are dissolved.
Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl.
Note: Prepare the staining solution shortly before use.
Preparation of Additional Solutions Required
Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven.
Information Note: The solution remains turbid.
Bring the stock solution to 15 to 25 °C until all components are dissolved.
Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl.
Note: Prepare the staining solution shortly before use.
Preparation of Additional Solutions Required
Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven.
Information Note: The solution remains turbid.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Rahul Satija et al.
Nature biotechnology, 33(5), 495-502 (2015-04-14)
Spatial localization is a key determinant of cellular fate and behavior, but methods for spatially resolved, transcriptome-wide gene expression profiling across complex tissues are lacking. RNA staining methods assay only a small number of transcripts, whereas single-cell RNA-seq, which measures
Practical Methods in Cardiovascular Research
Dhein S
Springer Science & Business Media (2006)
Anna-Carina Weiss et al.
Development (Cambridge, England), 141(17), 3420-3430 (2014-08-21)
The vesico-ureteric junction (VUJ) forms through a complex developmental program that connects the primordium of the upper urinary tract [the nephric duct (ND)] with that of the lower urinary tract (the cloaca). The signals that orchestrate the various tissue interactions
Federico Tessadori et al.
eLife, 10 (2021-08-11)
Organ laterality refers to the left-right asymmetry in disposition and conformation of internal organs and is established during embryogenesis. The heart is the first organ to display visible left-right asymmetries through its left-sided positioning and rightward looping. Here, we present
Peichao Li et al.
Carcinogenesis, 42(1), 136-147 (2020-07-28)
Hexavalent chromium [Cr(VI)] is a potent human lung carcinogen. Multiple mechanisms have been proposed that contribute to Cr(VI)-induced lung carcinogenesis including oxidative stress, DNA damage, genomic instability and epigenetic modulation. However, the molecular mechanisms and pathways mediating Cr(VI) carcinogenicity have
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
