11585029001

NBT

crystals, pkg of 5 g

• Synonim(y): Nitrotetrazolium Blue chloride, 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
• Wzór liniowy: C₄₀H₃₀N₁₀O₆ · 2Cl
• Numer CAS: 298-83-9
• Masa cząsteczkowa: 817.64
• Beilstein: 4115923
• Numer MDL: MFCD00012159
• Kod UNSPSC: 41116100
• Identyfikator substancji w PubChem: 329749491

Właściwości

• opis: 4-Nitro blue tetrazolium chloride, crystals
• Poziom jakości: 100
• Formularz: crystals
• opakowanie: pkg of 5 g
• producent / nazwa handlowa: Roche
• temp. przechowywania: 2-8°C
• ciąg SMILES: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• Klucz InChI: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Opis

Opis ogólny

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.[1] In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI.[2] Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications.[1] It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.[3]

Zastosowanie

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization[4]
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.[5]

Uwaga dotycząca przygotowania

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Komentarz do analizy

Kontrola wydajności w teście LDH.

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H302,H315,H319,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash