Wszystkie zdjęcia(1)
Kluczowe dokumenty
11544675001
Roche
Test ELISA do wykrywania śmierci komórek
sufficient for ≤96 tests, suitable for ELISA, kit of 1 (9 components)
Synonim(y):
ELISA
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wybierz wielkość
96 TESTS
3210,00 zł
3210,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Wybierz wielkość
Zmień widok
96 TESTS
3210,00 zł
About This Item
Kod UNSPSC:
41116158
3210,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
zastosowanie
sufficient for ≤96 tests
Poziom jakości
opakowanie
kit of 1 (9 components)
producent / nazwa handlowa
Roche
metody
ELISA: suitable
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
The Cell Death Detection ELISA serves as photometric enzyme immunoassay for the qualitative and quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death. This assay is based on the quantitative sandwich-enzyme immunoassay-principle using mouse monoclonal antibodies directed against DNA and histones, respectively. The antibodies used are not species specific.
Specyficzność
Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, and Xenopus). Anti-DNA-POD antibody binds to ss- and dsDNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.
Zastosowanie
Cell Death Detection ELISA has been used to measure Caspase-3 activity, intracellular reactive oxygen species (ROS) and cell apoptosis.[1]
Opakowanie
1 kit containing 9 components.
Uwaga dotycząca przygotowania
Working solution: Coating solution
Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer.
Washing solution
Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly.
Sample solution
Preparation of the sample solution depends on the cell system used and the extent of cell death.
Example: Dilute 25 μl of sample in 225 μl Incubation buffer.
Conjugate solution
Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer.
Substrate solution
Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer.
Allow to come to 15 to 25 °C before use.
Note: The ABTS solution is light sensitive over extended periods of time.
Storage conditions (working solution): Anti-histone
2 to 8 °C for 2 months
Anti-DNA-POD
2 to 8 °C for 2 months
Coating solution
Prepare immediately before use!
Washing solution
2 to 8 °C for 2 months
Sample solution
2 to 8 °C for 2 months
Conjugate solution
Prepare immediately before use!
Substrate solution
2 to 8 °C for 1 month, protected from light.
Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer.
Washing solution
Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly.
Sample solution
Preparation of the sample solution depends on the cell system used and the extent of cell death.
Example: Dilute 25 μl of sample in 225 μl Incubation buffer.
Conjugate solution
Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer.
Substrate solution
Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer.
Allow to come to 15 to 25 °C before use.
Note: The ABTS solution is light sensitive over extended periods of time.
Storage conditions (working solution): Anti-histone
2 to 8 °C for 2 months
Anti-DNA-POD
2 to 8 °C for 2 months
Coating solution
Prepare immediately before use!
Washing solution
2 to 8 °C for 2 months
Sample solution
2 to 8 °C for 2 months
Conjugate solution
Prepare immediately before use!
Substrate solution
2 to 8 °C for 1 month, protected from light.
Rekonstytucja
Anti-histone
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly.
Anti-DNA-POD
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly.
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly.
Anti-DNA-POD
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Tylko elementy zestawu
Numer produktu
Opis
- Anti-histone antibody (clone H11–4)
- Anti-DNA-POD antibody (clone MCA-33)
- Coating Buffer
- Washing Buffer
- Incubation Buffer, ready-to-use solution
- Substrate Buffer, ready-to-use solution
- ABTS Substrate Tablet
- Microplate Modules
- Adhesive Plate Cover Foils
Zobacz wszystko (9)
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Dam. 1 - Skin Sens. 1
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Alice Elizabeth Kane et al.
Fundamental & clinical pharmacology, 30(3), 263-275 (2016-01-29)
Paracetamol is an analgesic commonly used by people of all ages, which is well documented to cause severe hepatotoxicity with acute overexposures. The risk of hepatotoxicity from nonacute paracetamol exposures is less extensively studied, and this is the exposure most
Silvia Di Loreto et al.
The international journal of biochemistry & cell biology, 40(2), 245-257 (2007-09-18)
The hippocampus is known to play a crucial role in learning and memory. Recent data from literature show that cognitive problems, common to aged or diabetic patients, may be related to accumulation of toxic alpha-oxoaldehydes such as methylglyoxal. Thus, it
Allyson G Hindle et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 155(4), 427-434 (2010-01-30)
Red-toothed shrews (Soricidae, subfamily Soricinae) are an intriguing model system to examine the free-radical theory of aging in wild mammals, given their short (<18months) lifespan and high mass-specific metabolic rates. As muscle performance underlies both foraging ability and predator avoidance
Hua He et al.
Scientific reports, 7, 43736-43736 (2017-03-03)
Proliferative vitreoretinopathy (PVR) is mediated by proliferation and epithelial mesenchymal transition (EMT) of retinal pigment epithelium (RPE). Because heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) purified from human amniotic membrane exerts anti-inflammatory and anti-scarring actions, we hypothesized that HC-HA/PTX3 could inhibit these
J P Heiserman et al.
Cell stress & chaperones, 20(3), 527-535 (2015-02-27)
Extracellular (ex) HSP60 is increasingly recognized as an agent of cell injury. Previously, we reported that low endotoxin exHSP60 causes cardiac myocyte apoptosis. Our findings supported a role for Toll-like receptor (TLR) 4 in HSP60 mediated apoptosis. To further investigate
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
