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ABC118

Sigma-Aldrich

Anti-phospho Beclin Antibody (Thr119)

from rabbit, purified by affinity chromatography

Synonim(y):

Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

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Wybierz wielkość

100 μG
1880,00 zł

1880,00 zł

Przewidywany termin wysyłki22 kwietnia 2025Szczegóły

Wszystkie zdjęcia(2)

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Zmień widok
100 μG
1880,00 zł

About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

1880,00 zł

Przewidywany termin wysyłki22 kwietnia 2025Szczegóły

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

human

reaktywność gatunkowa (przewidywana na podstawie homologii)

rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)

metody

inhibition assay: suitable (peptide)
western blot: suitable

numer dostępu NCBI

NP_003757

numer dostępu UniProt

Q14457

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

phosphorylation (pThr119)

informacje o genach

human ... BECN1(8678)

Opis ogólny

The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

Specyficzność

This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.

Immunogen

Epitope: BH3 domain
KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.

Zastosowanie

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.

Jakość

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.

Opis wartości docelowych

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Postać fizyczna

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Human prostate tumor tissue lysate.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Miray Turk et al.
Molecules (Basel, Switzerland), 26(4) (2021-02-11)
Expression levels of the major mammalian autophagy regulator Beclin 1 and its interaction with Bcl-2 regulate the switch between autophagic cell survival and apoptotic cell death pathways. However, some of the regulators and the precise mechanisms of these processes still
Changxuan Chen et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 51(6), e7080-e7080 (2018-04-26)
Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of
M-L Plissonnier et al.
Oncogene, 36(48), 6712-6724 (2017-08-08)
Hepatitis C virus (HCV) infection is a leading cause of hepatocellular carcinoma (HCC), mainly through cirrhosis induction, spurring research for a deeper understanding of HCV versus host interactions in cirrhosis. The present study investigated crosstalks between HCV infection and UNC5A
Hanan Abu-Tayeh Suleiman et al.
International journal of molecular sciences, 23(20) (2022-10-28)
The kidney glomerular filtration barrier (GFB) is enriched with heparan sulfate (HS) proteoglycans, which contribute to its permselectivity. The endoglycosidase heparanase cleaves HS and hence appears to be involved in the pathogenesis of kidney injury and glomerulonephritis. We have recently
David Mondaca-Ruff et al.
Frontiers in pharmacology, 9, 1553-1553 (2019-02-26)
Hypertension is a disease associated to increased plasma levels of angiotensin II (Ang II). Ang II can regulate proliferation, migration, ROS production and hypertrophy of vascular smooth muscle cells (VSMCs). However, the mechanisms by which Ang II can affect VSMCs
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