OK to freeze desiccated (hygroscopic) protect from light
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Opis ogólny
A potent and selective inhibitor of cyclin-dependent kinases (Cdks). Exhibits greater inhibitory potency compared to olomoucine (Cat. No. 495620). Inhibits p34 Cdc2/cyclin B (IC50 = 650 nM), p33 Cdk2/cyclin A (IC50 = 700 nM), p33 Cdk2/cyclin E (IC50 = 700 nM), and p33 Cdk2/p35 (IC50 = 200 nM) by competing for the ATP binding domain of these kinases. Exhibits very low sensitivity towards related kinases, such as Erk1 and Erk2 (IC50 = 34 µM and 14 µM, respectively). Roscovitine does not significantly affect the activity of other protein kinases even at 100 µM and compared to olomoucine, roscovitine displays increased anti-mitotic activity at the G1/S and G2/M phases of the cell cycle.
Działania biochem./fizjol.
Cell permeable: no
Primary Target p34cdk1/cyclin B
Product competes with ATP.
Reversible: yes
Target IC50: 650 nM, 700 nM, 700 nM, and 200 nM against p34cdk1/cyclin B, p33cdk2/cyclin A, p33cdk2/cyclin E, and p33cdk5/p35, respectively
Opakowanie
Packaged under inert gas
Ostrzeżenie
Toxicity: Irritant (B)
Postać fizyczna
A 50 mM (5 mg/282 µl) solution of Roscovitine (Cat. No. 557360) in DMSO.
Rekonstytucja
Following initial use, aliquot and freeze (-20°C). Once opened, the DMSO aliquots are stable for up to 6 months at -20°C.
Inne uwagi
Meijer, L. 1996. Trends Cell Biol.6, 393. Rudolph, B., et al. 1996. EMBO J.15, 3053.
Informacje prawne
Sold under license of U.S. Patent 6,316,456.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
188.6 °F - closed cup - (Dimethylsulfoxide)
Temperatura zapłonu (°C)
87 °C - closed cup - (Dimethylsulfoxide)
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