529625

Polyphosphoinositide-Binding Peptide, PBP10

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin.

• Synonim(y): Polyphosphoinositide-Binding Peptide, PBP10, RhoB-GS _160-169, Rhodamine B-QRLFQVKGRR-OH, RhoB-GS_160-169, Rhodamine B-QRLFQVKGRR-OH
• Wzór empiryczny (zapis Hilla): C₈₄H₁₂₇N₂₄O₁₅
• Numer CAS: 794466-45-8
• Masa cząsteczkowa: 1713.06
• Numer MDL: MFCD00026695
• Kod UNSPSC: 12352211
• NACRES: NA.77

Właściwości

• Poziom jakości: 100
• Próba: ≥95% (HPLC)
• Formularz: lyophilized solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic); protect from light
• kolor: pink-red
• rozpuszczalność: DMSO: 2 mg/mL
• fluorescencja: λ_ex 565 nm; λ_em 590 nm
• Warunki transportu: ambient
• temp. przechowywania: −20°C

Opis

Opis ogólny

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
Specifically binds to polyphosphoinositide (PPI)

Product does not compete with ATP.

Reversible: yes

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Standard Handling (A)

Sekwencja

Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH

Postać fizyczna

Supplied as a trifluoroacetate salt.

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Inne uwagi

Bucki, R., et al. 2001. Biochemistry40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable