480010-M

NDSB-256

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.

• Synonim(y): NDSB-256, Dimethylbenzylammonium Propane Sulfonate
• Wzór empiryczny (zapis Hilla): C₁₂H₁₉NO₃S
• Numer CAS: 81239-45-4
• Masa cząsteczkowa: 257.35
• Numer MDL: MFCD00225018
• Kod UNSPSC: 12161902
• NACRES: NA.21

Właściwości

• Poziom jakości: 100
• Próba: ≥99% (TLC)
• Postać: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic)
• kolor: white
• rozpuszczalność: water: 200 mg/mL
• temp. przechowywania: 10-30°C
• InChI: 1S/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3
• Klucz InChI: MEJASPJNLSQOAG-UHFFFAOYSA-N

Opis

Opis ogólny

A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

Ostrzeżenie

Toxicity: Irritant (B)

Rekonstytucja

Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.

Inne uwagi

Goldberg, M.E., et al. 1996. Folding & Design1, 21.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable