341583

FGF-2, Basic, Bovine

• Synonim(y): FGF-2, Basic, Bovine, nbbFGF, Fibroblast Growth Factor, Basic, Bovine
• Numer CAS: 106096-93-9
• Numer MDL: MFCD00081382
• Kod UNSPSC: 12352202

Właściwości

• Próba: ≥97% (SDS-PAGE)
• Poziom jakości: 100
• Postać: lyophilized
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• zanieczyszczenia: <0.1 ng/μg Endotoxin (ng/μg FGF)
• Warunki transportu: ambient
• temp. przechowywania: −20°C

Opis

Opis ogólny

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.

Działania biochem./fizjol.

ED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Lyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.

Rekonstytucja

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Inne uwagi

Ohmiya, M., et al. 2001. J. Neurosci. Res.65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol.147, 120.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3