16-202A

Przeciwciało anty-fosfo-histonowe H2A.X (Ser139), klon JBW301, koniugat FITC

clone JBW301, Upstate^®, from mouse

• Synonim(y): H2AXS139P, histon H2A.X (fosfo S139), rodzina histonów H2A, członek X, histon H2AX
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• białko sprzężone: FITC conjugate
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: JBW301, monoclonal
• reaktywność gatunkowa: human
• producent / nazwa handlowa: Upstate^®
• metody: flow cytometry: suitable; immunocytochemistry: suitable
• izotyp: IgG1
• numer dostępu NCBI: NM_002105.2
• numer dostępu UniProt: P16104
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer139)
• informacje o genach: human ... H2AX(3014)

Opis

Opis ogólny

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specyficzność

Broad species cross-reactivity is expected based on conservation of sequence homology.

Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

Immunogen

Epitope: Ser139

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

Zastosowanie

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton^™ X-100 in TBS (5 min.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

Jakość

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

Opis wartości docelowych

15 kDa

Postać fizyczna

Protein G Purified

Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

Przechowywanie i stabilność

Stable for 1 year at from date of receipt.

Komentarz do analizy

Control
Staurosporine-treated Jurkat cells

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable