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A2228

Sigma-Aldrich

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-74, purified immunoglobulin, buffered aqueous solution

Synonim(y):

Actin Antibody Sigma, Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse, Beta Actin Antibody Sigma, Beta Actin Sigma Antibody, Sigma Actin Antibody, Sigma Beta Actin Antibody

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About This Item

Numer MDL:
MFCD00164531
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

AC-74, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen 42 kDa

reaktywność gatunkowa

rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human

spodziewany brak reakcji z

Dictyostelium discoideum

stężenie

~2 mg/mL

metody

immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts
immunohistochemistry: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts

izotyp

IgG2a

numer dostępu UniProt

P60709

Zastosowanie

research pathology

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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Opis ogólny

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.
Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Zastosowanie

Monoclonal Anti-β-Actin antibody has been used in western blot and two-dimensional gel immunoblot.
Monoclonal anti-β-actin antibody can be used for immunocytochemistry (10-40μg/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Działania biochem./fizjol.

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Postać fizyczna

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Immune Pathology Associated with Altered Actin Cytoskeleton Regulation.
Dilki W, et al.
Autoimmunity, 43(1), 64-75 (2010)
β-Actin specifically controls cell growth, migration, and the G-actin pool
Tina M B, et al.
Molecular Biology of the Cell, 22(21) (2011)
Yuyu Yang et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(8), 834-845 (2019-06-04)
Prostate cancer malignancies are intimately correlated with deregulated fatty acid metabolism. The underlying epigenetic mechanism is not fully understood. In the present study we investigated the mechanism whereby the chromatin remodeling protein BRG1 regulates the transcription of long-chain fatty acid
Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells
Alexandra CS, et al.
Cancer Letters, 276(1), 32-37 (2009)
Baicalein Reduces Inflammatory Process in a Rodent Model of Diabetic Retinopathy.
Yang L.P, et al.
Investigative Ophthalmology & Visual Science, 50(5), 2319-2327 null
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