05-1307-AF488
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor™ 488 conjugate
clone Apu2, from rabbit, ALEXA FLUOR™ 488
Synonim(y):
Polyubiquitin-B, Ubiquitin, Lys48-Specific, Ubiquitin
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
białko sprzężone
ALEXA FLUOR™ 488
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
Apu2, monoclonal
reaktywność gatunkowa
rat, mouse, human
reaktywność gatunkowa (przewidywana na podstawie homologii)
rabbit (based on 100% sequence homology)
metody
immunocytochemistry: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... UBB(7314)
mouse ... Ubg(107729)
Opis ogólny
Polyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
Specyficzność
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
Immunogen
Epitope: Lys48 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys48.
Zastosowanie
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor™ 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.
Jakość
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.
Opis wartości docelowych
~17 kDa observed
Postać fizyczna
Protein A purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Informacje prawne
ALEXA FLUOR is a trademark of Life Technologies
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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