Przejdź do zawartości
Merck
Wszystkie zdjęcia(6)

Kluczowe dokumenty

Wyświetl całą dokumentację

MAB374

Sigma-Aldrich

Anti-GAPDH Antibody

CHEMICON®, mouse monoclonal, 6C5

Synonim(y):

glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, glyceraldehyde-3-phosphate dehydrogenase, GAPDH, G3PDH, GAPD, OK/SW-cl.12, MGC88685, G3PD, EC 1.2.1.12

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(6)

About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
6C5, monoclonal
application:
ELISA
ICC
IF
IHC
IP
WB
reaktywność gatunkowa:
human, feline, pig, mouse, rabbit, fish, canine, rat
metody:
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
3155

Nazwa produktu

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5, clone 6C5, Chemicon®, from mouse

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

6C5, monoclonal

reaktywność gatunkowa

human, feline, pig, mouse, rabbit, fish, canine, rat

spodziewany brak reakcji z

E. coli

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1

numer dostępu NCBI

NP_002037

numer dostępu UniProt

P04406

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Opis ogólny

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

Specyficzność

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle. It has been reported that this antibody does not react with GAPDH from E. coli.

Immunogen

Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.

Zastosowanie

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
ELISA:
1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

Immunocytochemistry:
1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

Western blot:
1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

Immunoaffinity purification of GAPDH.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry

Jakość

Evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.

Opis wartości docelowych

38 kDa

Postać fizyczna

Format: Purified
Protein A purified
Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.

Przechowywanie i stabilność

Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.

Komentarz do analizy

Control
It is expressed in all cells.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

polecane

Numer produktu
Opis
Cennik

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Kristina Leuner et al.
PloS one, 6(2), e14716-e14716 (2011-03-03)
Psoriasis is a characteristic inflammatory and scaly skin condition with typical histopathological features including increased proliferation and hampered differentiation of keratinocytes. The activation of innate and adaptive inflammatory cellular immune responses is considered to be the main trigger factor of
Anti-VEGF antibody treatment accelerates polycystic kidney disease.
Raina, S; Honer, M; Kramer, SD; Liu, Y; Wang, X; Segerer, S; Wuthrich, RP; Serra, AL
American Journal of Physiology: Renal Physiology null
Manoj B Menon et al.
PloS one, 6(8), e23054-e23054 (2011-08-20)
SB202190, a widely used inhibitor of p38 MAPKα and β, was recently described to induce autophagic vacuoles and cell death in colon and ovarian cancer cells lines and, therefore, this effect was supposed to be specific for transformed cells and
An integrated systems analysis implicates EGR1 downregulation in simian immunodeficiency virus encephalitis-induced neural dysfunction.
Gersten, M; Alirezaei, M; Marcondes, MC; Flynn, C; Ravasi, T; Ideker, T; Fox, HS
The Journal of Neuroscience null
Studying protein degradation pathways in vivo using a cranial window-based approach.
Unni, VK; Ebrahimi-Fakhari, D; Vanderburg, CR; McLean, PJ; Hyman, BT
Methods null
Wyświetl wszystkie powiązane dokumenty

Produkty

Wskazówki dotyczące protokołów immunofluorescencji

Immunofluorescencja wykorzystuje cząsteczki fluorescencyjne sprzężone z przeciwciałami do lokalizacji białek, potwierdzania modyfikacji i wizualizacji kompleksów białkowych.

Tips for Immunofluorescence Protocols

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

Protokoły

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej