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等级
for molecular biology
描述
nutrient rich, quick dissolve, dust free formulation
无菌性
non-sterile
表单
powder
组成
Inert binder (EZMix only), 0.6 g/L
NaCl, 5 g/L
Tryptone (pancreatic digest of casein), 16 g/L
Yeast extract, 10 g/L
应用
agriculture
food and beverages
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
应用
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
特点和优势
2xYT EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Easy scale-up using larger package size
- Standard formulation
重悬
Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
法律信息
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
其他客户在看
Shuang Yang et al.
eLife, 9 (2020-06-09)
Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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