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Merck
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文件

L7533

Sigma-Aldrich

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

别名:

LB broth

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

品質等級

200

無菌

non-sterile

形狀

powder

成份

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

技術

microbiological culture: suitable

應用

agriculture
food and beverages
microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

應用

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

特點和優勢

Lennox EZMix powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

準備報告

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

重構

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

法律資訊

Zeocin is a registered trademark of Cayla Sarl

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儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Sambrook, J., et al
Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
Ausubel, F. M.
Current Protocols in Molecular Biology, 1, 3-3 (1994)
Alexis Kaushansky et al.
Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
Shuang Wang et al.
STAR protocols, 2(2), 100440-100440 (2021-04-27)
Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into
Raymond Liang et al.
Cell stem cell, 26(3), 359-376 (2020-02-29)
Quiescence is a fundamental property that maintains hematopoietic stem cell (HSC) potency throughout life. Quiescent HSCs are thought to rely on glycolysis for their energy, but the overall metabolic properties of HSCs remain elusive. Using combined approaches, including single-cell RNA

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