跳转至内容
Merck

L3022

Sigma-Aldrich

LB 肉汤(Lennox)

Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

别名:

Luria Bertani肉汤, 伦诺克斯肉汤

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

無菌

non-sterile

形狀

powder

成份

NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

技術

microbiological culture: suitable

pH值

6.8-7.2(2% solution)

應用

food and beverages
microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。

應用

Luria肉汤被用于大肠杆菌(Escherichia coli)的维持和繁殖。 大肠杆菌在Luria肉汤中生长得更快,因为胰蛋白胨和酵母为其提供了必需的生长因子,从而无需大肠杆菌自身合成。由于含有NaCl成分,Luria肉汤还含有转运和渗透平衡所必需的电解质。
在三鞭毛虫细胞提取物的分级研究中,它被用于梨形四膜虫胞级分的重悬。
适用于 大肠杆菌 菌株的非选择性培养,用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养,包括需要低盐条件的抗生素,如吉欧霉素®

特點和優勢

Lennox LB粉末可提供:
  • 便捷式小包装,无需称重步骤
  • 使用大包装可轻松放大规模
  • 一种预算较低的液体替代品
  • 标准配方

準備報告

1.将20 g悬浮于1L蒸馏水中。
2.121 °C下高压灭菌15分钟。
制备LB肉汤:添加1 g葡萄糖,然后按照上述说明操作。
制备Enquist &Sternberg培养基:高压蒸汽灭菌后,以无菌操作加入10 mL无菌1M硫酸镁。

法律資訊

Zeocin is a registered trademark of Cayla Sarl

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

J Benavente et al.
International journal of biological macromolecules, 103, 758-763 (2017-05-27)
The preparation of silver nanoparticles (AgNPs) and their incorporation into the structure of a regenerated cellulose membrane by dip coating is presented. Morphological characterization of the AgNPs (average diameter of 20±2nm) was carried out by SEM/TEM, while elastic, electrical and
Francisco Dávila et al.
The Journal of experimental biology, 221(Pt 6) (2018-02-16)
Bacteria can damage sperm and thus reduce the reproductive success of both males and females; selection should therefore favour the evolution of antimicrobial protection. Eusocial hymenopterans might be particularly affected by such bacterial infections because of their mating ecology. In
Margot Doberva et al.
Frontiers in microbiology, 8, 1152-1152 (2017-07-12)
Quorum sensing (QS) is a density-dependent mechanism allowing bacteria to synchronize their physiological activities, mediated by a wide range of signaling molecules including
Víctor González-Alonso et al.
Poultry science, 99(1), 536-545 (2020-05-18)
The objective of the present study was to assess the potential synergistic effect between supercritical carbon dioxide (SC-CO2) and fresh culinary herbs (Coriandrum sativum and Rosmarinus officinalis) on the microbial inactivation of raw chicken meat. The microbiological inactivation was performed
Bor Kavčič et al.
Nature communications, 11(1), 4013-4013 (2020-08-13)
Antibiotics that interfere with translation, when combined, interact in diverse and difficult-to-predict ways. Here, we explain these interactions by "translation bottlenecks": points in the translation cycle where antibiotics block ribosomal progression. To elucidate the underlying mechanisms of drug interactions between

实验方案

General protocols for growth of competent cells in microbial medium.

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门