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S1797

Sigma-Aldrich

SOC培养基

For use in transformation

别名:

Super Optimal broth

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About This Item

UNSPSC代码:
41106200
NACRES:
NA.85

等级

Molecular Biology
for molecular biology

无菌性

0.2 μm filtered

表单

liquid

组成

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

技术

microbiological culture: suitable

应用

agriculture

储存温度

2-8°C

适用性

nonselective for Escherichia coli
nonselective for coliforms

相关类别

一般描述

含有分解代谢抑制物的培养基（SOC）是一种富含葡萄糖的微生物生长培养基，主要用于大肠杆菌感受态细胞转化后的复苏。 在这种营养丰富的微生物培养基中培养大肠杆菌细胞，可以抑制克隆载体上携带的lac启动子的意外激活，从而提高含有毒蛋白基因的重组质粒的转化效率。SOC培养基采用低盐配方，含有多肽、氨基酸、水溶性维生素和葡萄糖。

应用

SOC培养基可用于大肠杆菌DH5α 感受态细胞的转化。 它还被用于培养克隆后的白色菌落，以鉴定感兴趣的重组蛋白。

特点和优势

SOC液体提供：
  • 经过滤灭菌的即用型培养基
  • 便利的包装规格
  • 标准配方

相关产品

产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCN8706
SLCL9800
SLCN2539
SLCF7416
SLCG7766

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Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
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