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Merck

L2542

Sigma-Aldrich

LB 肉汤

Liquid microbial growth medium

别名:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

無菌

sterile; 0.2 μm filtered

形狀

liquid

技術

microbiological culture: suitable

pH值

6.5-7.2

應用

microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

應用

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

特點和優勢

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

成分

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Celine Abueva et al.
International journal of biological macromolecules, 182, 1713-1723 (2021-05-30)
The importance of developing more potent antimicrobials and robust infection prevention practices has been highlighted recently with the increase in reports of emerging bacterial resistance mechanisms and the development of antibiotic-resistant microbes. In this study, a quaternary ammonium chitosan derivative
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth
Zhao LH, et al.
Sensors and actuators A, Physical, 181.0, 33-42 (2012)
Development of mesoporous borosilicate bioactive glass nanoparticles containing Mg2+ and Zn2+: biocompatibility, bioactivity and antibacterial activity
Damian-Buda A I
Journal of Non-Crystalline Solids, 594, 121819-121819 (2022)
Andrada-Ioana Damian-Buda et al.
Journal of functional biomaterials, 13(4) (2022-10-25)
Mesoporous bioactive glass nanoparticles (MBGNs) are widely recognized for their ability to bond to hard tissue, while the ions released from the BG structure enhance specific cellular pathways. In this study, the SiO2-P2O5-CaO-MgO-ZnO system was used to successfully synthesize MBGNs

商品

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

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实验方案

General protocols for growth of competent cells in microbial medium.

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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