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Key Documents

Y1003

Sigma-Aldrich

2x YT 培养基

Liquid microbial growth medium

别名:

2xTY培养基, 2xYT 培养基, 2x酵母提取物胰蛋白胨培养基

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About This Item

分類程式碼代碼:
41106225
NACRES:
NA.85

等級

for molecular biology

無菌

sterile; 0.2 μm filtered

形狀

liquid

技術

microbiological culture: suitable

pH值

6.5-7.5

應用

microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

2xYT(2x酵母提取物胰蛋白胨培养基)是用于培养大肠杆菌和M13噬菌体的标准微生物生长培养基。 这种营养丰富的微生物肉汤含有低盐配方中的多肽、氨基酸和水溶性维生素。

應用

2x YT培养基已应用于M13噬菌体的非选择性培养,以及克隆、DNA质粒生产和重组蛋白生产的大肠杆菌菌株。 当加入适当抗生素时,也适用于选择性培养。

特點和優勢

2xYT 液体提供:
  • 过滤灭菌,即用型形式
  • 方便的包装尺寸
  • 标准配方

成分

16 g/L 胰蛋白胨
10 g/L 酵母提取物
5.0 g/L NaCl

相關產品

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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