C8740 does not interfere with downstream applications such as 6X HisTag affinity tag purification, or detection on a Western Blot with an anti-6X His antibody.
If the intention is to conjugate a protein to a 6X HisTag, the protein should first be purified from the lysate before conjugation. C8740 will not interfere with a conjugation protocol after the protein of interest has been isolated from the lysate.
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应用
两性离子洗涤剂的专利、非变性配方，用于细菌细胞裂解和重组蛋白提取。
特点和优势
- 比传统方法如超声法和溶菌酶法提取蛋白质效率高
- 可放大 1-25 克细菌细胞浆
- 不干扰下游应用，如亲和层析、IP 和免疫印迹
- 与蛋白酶抑制剂、抑制剂混合物、离液剂、盐、螯合剂、还原剂相容
其他说明
洗涤剂混合物的 10X 浓缩液不含缓冲成分，使研究者能够灵活地将裂解试剂整合到其所需的系统中。重组蛋白提取和纯化的自动化方案也适用。
法律信息
此产品受美国专利号7,282,475 B2管辖，仅针对研究用途出售。如需商业化使用，需另行征得许可。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Can this product safe to be used for downstream crosslinking stains such as a 6x HIsTag?
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Can I use the CelLytic™ B cell lysate in an activity assay?
1 answer-
CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.
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When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
1 answer-
When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
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What bacterial cells can be used with CelLytic B?
1 answer-
CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
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Can I concentrate my sample that was made using CelLytic B?
1 answer-
If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
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What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
1 answer-
CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
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Can I obtain protein from inclusion bodies with CelLytic B?
1 answer-
If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use CelLytic B for eukaryotic cells?
1 answer-
CelLytic B was optimized for bacterial cells. It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.
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Can I use CelLytic B with the maltose binding protein system (MBP System)?
1 answer-
CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
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