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Merck
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S8830

Sigma-Aldrich

Protease Inhibitor Cocktail

SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins

别名:

Protease Inhibitor Cocktail

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About This Item

MDL號碼:
MFCD00677817
分類程式碼代碼:
12352200
NACRES:
NA.77

product name

SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins

生物源

bovine lung

形狀

tablet

儲存溫度

2-8°C

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相关类别

一般說明

SIGMAFAST™ 蛋白酶抑制剂混合液片，EDTA-Free是一种水溶性片剂配方，旨在抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。 该片剂不含强金属螯合剂，如EDTA，并与固定化金属亲和层析（IMAC）样品处理兼容。 该片剂还含有独特的专利配方，包括胃酶抑素A盐，以提供额外的保护。

特異性

抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。

應用

SIGMAFAST ™蛋白酶抑制剂混合液片的广泛特异性 使其适合用于组氨酸标记蛋白的纯化， 并作为通用蛋白酶抑制剂混合液。 该片剂还可以用作细胞裂解缓冲液的组件， 并已与HIS-Select介质一起使用进行了优化和测试。

特點和優勢

EDTA-Free： 不含可能干扰IMAC处理的强金属螯合剂，如EDTA。

胃酶抑素A：含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。

广泛特异性：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，为蛋白质样品提供广泛的保护。

高产率：一片可制成100 mL混合液，推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。

长期稳定性：该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液（1´或10´）在2–8 °C下至少可稳定保存2周。

成分

AEBSF
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽

數量

一片可制备100 mL混合液。 推荐将一片用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。

外觀

片剂是可溶于水的

儲存和穩定性

该片剂在2-8°C温度下至少可稳定保存2年。重悬的蛋白酶抑制剂溶液（1´或10´）在2–8°C下至少可稳定保存2周。

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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产品编号
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象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H314

危險分類

Eye Dam. 1 - Skin Corr. 1A

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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