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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

别名:

蛋白酶抑制剂溶液

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About This Item

EC號碼:
200-664-3
MDL號碼:
MFCD00677817
分類程式碼代碼:
12352200
NACRES:
NA.77
形狀:
DMSO solution
儲存溫度:
−20°C

产品名称

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

品質等級

200

形狀

DMSO solution

運輸包裝

dry ice

儲存溫度

−20°C

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相关类别

一般說明

蛋白酶抑制剂复合物混合多种抑制剂， 防止组织培养基中的分泌蛋白被蛋白酶降解。
蛋白酶抑制剂混合物处理以下细胞系48小时后无毒性：
A431、CHO、COS、HepG2和HeLa贴壁细胞系
Jurkat和HL-60悬浮细胞系

特異性

抑制丝氨酸、半胱氨酸、和天冬氨酸蛋白酶和氨肽酶

應用

本品 专门针对组织培养基优化， 在培养基中作用48小时后可换新液，确保持续性 抑制蛋白酶。
蛋白酶抑制剂混合物的应用示例：
  • 作为补充剂掺入上清液和裂解物，用于人精密切割肺切片(PCLS)的离体感染实验
  • 用于抑制胰蛋白酶介导的蛋白水解，证明蛋白水解可恢复被蛋白冠抑制的纳米酶的催化活性
  • 加入胰蛋白酶溶液用于胰蛋白酶抑制研究
  • 作为放射性免疫沉淀分析(RIPA)缓冲液组分，裂解白细胞用于蛋白质印迹分析

生化／生理作用

蛋白酶抑制剂混合物专用于组织培养基。适合作为组织培养基添加剂，保护培养的组织分泌蛋白免遭降解。

特點和優勢

  • 广泛的特异性
  • 无毒性：作用48小时后，本品对贴壁细胞系A431、CHO、COS、HepG2和HeLa，以及悬浮细胞系Jurkat和HL-60无毒。
  • 不含金属螯合剂：确保兼容下游应用
  • 方便包装：1 mL玻璃瓶包装
  • 易于使用：在组织培养基中以1：200或更高的稀释度使用，以防止分泌蛋白被蛋白酶水解。

成分

抑肽酶
抑氨肽酶
E-64
亮肽素
胃蛋白酶抑制剂A

注意

孵育48小时后，产物对贴壁细胞系A431、CHO、COS、HepG2和HeLa，以及悬浮培养的Jurkat和HL-60细胞系无毒。

數量

在组织培养基中以1：200或更高的稀释度使用，以防止分泌蛋白的蛋白水解降解。

外觀

溶于DMSO中（D 2650，Hybri-Max）。

其他說明

仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

188.6 °F

閃點（°C）

87 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000327277
0000182627
0000198327
019M4189V
029M4044V

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