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P8465

Sigma-Aldrich

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases, for use with bacterial cell extracts, lyophilized powder

别名:

Protease inhibitor

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77
形狀:
lyophilized powder
儲存溫度:
−20°C

产品名称

蛋白酶抑制剂混合物粉末, for use with bacterial cell extracts, lyophilized powder

品質等級

300

形狀

lyophilized powder

儲存溫度

−20°C

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相关类别

一般說明

蛋白酶抑制剂混合液是一种用于抑制存在于细菌细胞提取物中的蛋白酶的冻干粉末。

该产品含有针对丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶以及氨基肽酶的单独组分。

應用

蛋白酶抑制剂混合物，通过添加相关酶（细胞提取中存在的能够修饰蛋白质的酶）的抑制剂，来提高完整蛋白质的产量。 本产品已针对细菌细胞使用进行了优化和测试，对丝氨酸、半胱氨酸和天冬氨酸蛋白酶，金属蛋白酶和氨肽酶具有广泛的特异性。

生化／生理作用

该混合物含有单独的组分，包括 23mM 的 AEBSF，100mM 的 EDTA，2mM 的苯丁抑制素，0.3mM 的蛋白酶抑制剂 A 和 0.3mM 的 E-64。 AEBSF 用于抑制丝氨酸蛋白酶，包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。 苯丁抑制素抑制氨肽酶。 E-64 抑制半胱氨酸蛋白酶。 胃蛋白酶抑制剂 A 抑制酸性蛋白酶。 EDTA 是金属蛋白酶的抑制剂。

特點和優勢

对丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶以及氨基肽酶具有广泛的特异性。

含有单独的成分，包括AEBSF、EDTA、Bestatin、Pepstatin A和E-64，分别靶向特定类型的蛋白酶。

冻干粉在-20°C不开封时至少可稳定保存2年。

附一小瓶DMSO，用于制备混合溶液。

推荐使用1mL的混合溶液抑制4g大肠杆菌细胞的20mL细胞裂解物中的蛋白酶活性。

注意

未开封的冻干粉可在 -20℃ 下稳定储存至少 2 年。它还配有一瓶 DMSO。 在抑制剂沉淀出来之前，在 4℃ 下，制备的 DMSO 和水溶液可保持澄清无色约 24 小时。

準備報告

可以通过添加 1mL DMSO 震荡一分钟后溶解，再添加 4mL 去离子水稀释，最终得到澄清溶液；或者添加 5mL DMSO 震荡一分钟后溶解，再添加 20mL 去离子水稀释，最终得到澄清溶液。 推荐使用 1mL 的混合溶液抑制 4g 大肠杆菌细胞的 20mL 细胞裂解物中的蛋白酶活性。

相關產品

产品编号
说明
价格

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蛋白酶抑制剂混合物粉末, for general use, lyophilized powder

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S8820

SIGMAFAST™蛋白酶抑制剂片剂, For General Use

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SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000355404
0000303638
0000355404
0000303638
0000213110

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Ryan W Bogard et al.
mBio, 3(5), e00236-e00212 (2012-09-28)
LysR-type transcriptional regulators (LTTRs) are the largest, most diverse family of prokaryotic transcription factors, with regulatory roles spanning metabolism, cell growth and division, and pathogenesis. Using a sequence-defined transposon mutant library, we screened a panel of V. cholerae El Tor
H Cao et al.
Plant physiology, 120(1), 205-216 (1999-05-11)
This study identified the complement of soluble starch synthases (SSs) present in developing maize (Zea mays) endosperm. The product of the du1 gene, DU1, was shown to be one of the two major soluble SSs. The C-terminal 450 residues of
Evgeniy V Petrotchenko et al.
Molecular & cellular proteomics : MCP, 11(7), M111-M111 (2012-03-23)
Chemical cross-linking combined with mass spectrometry is a rapidly developing technique for structural proteomics. Cross-linked proteins are usually digested with trypsin to generate cross-linked peptides, which are then analyzed by mass spectrometry. The most informative cross-links, the interpeptide cross-links, are
Joel Berry et al.
Molecular microbiology, 70(2), 341-351 (2008-08-21)
Bacteriophage lambda has four adjacent genes -S, R, Rz and Rz1- dedicated to host cell lysis. While S, encoding the holin and antiholin, and R, encoding the endolysin, have been intensively studied, the products of Rz and Rz1 have not
Kerri Kobryn et al.
Molecular cell, 9(1), 195-201 (2002-01-24)
The genus Borrelia includes the causative agents of Lyme disease and relapsing fever. An unusual feature of these bacteria is a segmented genome consisting mostly of a number of linear DNA molecules with covalently closed hairpin ends or telomeres. In
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