跳转至内容
Merck
所有图片(1)

文件

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

别名:

Protease inhibitor solution

登录查看公司和协议定价
所有图片(1)

About This Item

MDL號碼:
MFCD00677817
分類程式碼代碼:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

生物源

synthetic

品質等級

300

形狀

DMSO solution

儲存溫度

−20°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。

溶液中混有多种抑制剂成分，可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。

特異性

抑制丝氨酸，半胱氨酸，天冬氨酸，和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

應用

混合液经过专门的优化和验证，适合分离组氨酸标签(His-tag)蛋白质，特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。

推荐用量：1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。

特點和優勢

专门为组氨酸标签蛋白纯化而配制。

靶向多种蛋白酶，具有全面的蛋白酶抑制活性。

不加螯合剂，适合IMAC应用。

方便的包装选择。

成分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

數量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

準備報告

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

相關產品

产品编号
说明
价格

P8465

蛋白酶抑制剂混合物粉末, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

蛋白酶抑制剂混合物粉末, for general use, lyophilized powder

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8820

SIGMAFAST™蛋白酶抑制剂片剂, For General Use

S8830

SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

185.0 °F - closed cup

閃點（°C）

85 °C - closed cup

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Z Kurowska et al.
Neuroscience, 256, 456-466 (2013-10-26)
Nogo-A is a transmembrane protein originally discovered in myelin, produced by postnatal CNS oligodendrocytes. Nogo-A induces growth cone collapse and inhibition of axonal growth in the injured adult CNS. In the intact CNS, Nogo-A functions as a negative regulator of
E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of
Shuji Kaneko et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(1), 82-92 (2002-01-05)
The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
Ryan McKay et al.
Biotechnology and bioengineering, 114(12), 2883-2895 (2017-07-30)
Probiotics, whether taken as capsules or consumed in foods, have been regarded as safe for human use by regulatory agencies. Being living cells, they serve as "tunable" factories for the synthesis of a vast array of beneficial molecules. The idea
Thomas A Leonard et al.
Cell, 144(1), 55-66 (2011-01-11)
Protein kinase C (PKC) isozymes are the paradigmatic effectors of lipid signaling. PKCs translocate to cell membranes and are allosterically activated upon binding of the lipid diacylglycerol to their C1A and C1B domains. The crystal structure of full-length protein kinase
查看所有相关文献

相关内容

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门