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Merck
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文件

L4544

Sigma-Aldrich

Human Laminin

from human fibroblasts, cell culture derived, liquid, 0.5 mg/mL, suitable for cell culture

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About This Item

MDL號碼:
MFCD00081739
分類程式碼代碼:
12352202
NACRES:
NA.75

product name

层粘蛋白 来源于人类成纤维细胞, cell culture derived, liquid, sterile-filtered

生物源

human fibroblasts

品質等級

200

無菌

sterile-filtered

形狀

liquid

包裝

pkg of 100 μL

技術

cell culture | mammalian: suitable

表面覆盖率

1‑2 μg/cm2

UniProt登錄號

P07942

結合特異性

Peptide Source: Collagen

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... LAMB1(3912)

相关类别

應用

人成纤维细胞的层粘连蛋白用于：
  • 用于伤口愈合分析的六孔板涂层
  • 作为细胞粘附和扩散分析的底物
  • 用于人类胚胎干细胞(hESC)和人类诱导多能干细胞(hiPSC)向多巴胺能神经元的分化
  • 用于角膜内皮细胞伤口愈合(迁移)分析和角膜内皮细胞屏障分析

推荐使用人成纤维细胞的层粘连蛋白作为1-2 μg/cm2细胞培养基基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

生化／生理作用

层粘连蛋白是动物组织中结构性支架的组成部分。它们通过内功素和基底膜聚糖与IV型胶原结合，并通过整合素受体、dystroglycan糖蛋白复合物以及Lutheran血型糖蛋白与细胞膜结合。层粘连蛋白具有胶原结合、细胞粘附、肝素结合和神经突向外生长片段的活性结构域。层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。

成分

层粘连蛋白是细胞外基质多结构域三聚体糖蛋白，并且是支持粘附、增殖和分化的基底层的主要非胶原组分。 层粘连蛋白由A、B1和B2 链组成，它们通过许多二硫键连接。该层粘连蛋白产物由人成纤维细胞和上皮细胞在共培养系统中产生，然后通过生物化学方法纯化。

注意

建议将此产品储存在-70°C，它可以其中稳定保存两年。

準備報告

该产品在TBS中以 0.5mg/mL的浓度供应。在使用前在2-8°C下将该溶液缓慢解冻以避免形成凝胶。如果在解冻之前的任何时候过早加热层粘连蛋白，则可能形成凝胶。此外，即使在 -20C下储存也可能导致凝胶形成或活性显著下降，或出现这两种现象。用作包膜时，在HBSS中稀释，以最小体积涂覆培养物表面并在37°C 下孵育 1-2 小时。 在接种细胞前使用HBBS 洗涤 3 次。层粘连蛋白包膜可在2-8°C储存一个月。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
Lukovic D, et al.
Stem Cells Translational Medicine, 6(4), 1217-1226 (2017)
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Nunzia Di Maggio et al.
Scientific reports, 7, 44398-44398 (2017-03-16)
Stromal vascular fraction (SVF) cells of human adipose tissue have the capacity to generate osteogenic grafts with intrinsic vasculogenic properties. However, adipose-derived stromal/stem cells (ASC), even after minimal monolayer expansion, display poor osteogenic capacity in vivo. We investigated whether ASC
Elucidating the molecular basis of PPCD: Effects of decreased ZEB1 expression on corneal endothelial cell function
Zakharevich M, et al.
Molecular Vision, 740-740 (2017)
Serum-induced differentiation of glioblastoma neurospheres leads to enhanced migration/invasion capacity that is associated with increased MMP9
Joseph JV, et al
PLoS ONE, 10(12), e0145393-e0145393 (2015)
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