跳转至内容
Merck
所有图片(1)

文件

11243217001

Roche

层粘蛋白

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

别名:

层粘连蛋白 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤基底膜

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
114956-81-9
MDL號碼:
MFCD00081739
分類程式碼代碼:
12352207

生物源

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

品質等級

100

無菌

sterile; sterile-filtered

化驗

≥90% (SDS-PAGE)

形狀

solution

分子量

900 kDa

包裝

pkg of 1 mg (2 ml)

製造商／商標名

Roche

技術

cell culture | mammalian: suitable

UniProt登錄號

Q61292

儲存溫度

−20°C

基因資訊

mouse ... Lamb2(16779)

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

层粘连蛋白是从小鼠Engelbreth-Holm-Swarm (EHS)肉瘤的层粘连蛋白-巢蛋白复合物中分离得到的。层粘连蛋白是由α、β和γ 链亚基组成的异源三聚体。它是基底膜结构支架所需的糖蛋白。层粘连蛋白支持许多细胞类型的生长和分化，包括上皮细胞、内皮细胞、神经细胞、肌肉细胞和肝细胞。

生物活性：经测试可促进HT-1080细胞的贴壁。
特异性：对大多数哺乳动物细胞有活性。

應用

层粘连蛋白已经证明可促进多种细胞的附着和生长；可用于包被培养皿。它已被用于细胞贴壁测定。推荐以1-2μg/cm2用作细胞培养基质。最佳浓度取决于细胞类型以及应用或研究目标。
层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

品質

纯度：≥90% (SDS-PAGE) (作为层粘连蛋白巢蛋白复合物，1:1)

序列

小鼠层粘连蛋白由三条多肽链组成（A：400 kD，B1：230 kD和B2：220 kD），通过二硫键连接，并且是糖基化的。

外觀

溶液，0.5 mg/ml层粘连蛋白，溶剂为0.15 M NaCl，2 mM EDTA，0.05 M Tris-HCl，pH 7.4，无菌
溶液，通过0.2 ?m孔径滤膜过滤

準備報告

工作浓度：2至5 μg/cm2
2至5 μg/cm2 ，用于包被细胞培养容器。
储存条件（工作溶液）：2至8°C
未稀释溶液可在2至8°C下稳定保存至少3个月。
溶液应在2至8°C或冰上小心解冻。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

does not flash

閃點（°C）

does not flash

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Ahmad Abdullah et al.
Scientific reports, 11(1), 8585-8585 (2021-04-23)
Netrin-1 is a secreted protein that is well known for its involvement in axonal guidance during embryonic development and as an enhancer of cancer cell metastasis. Despite extensive efforts, the molecular mechanisms behind many of the physiological functions of netrin-1
Elias Theodorou et al.
Genes & development, 23(5), 575-588 (2009-03-10)
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced
查看所有相关文献

商品

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

查看所有结果

实验方案

用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门