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Merck
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主要文件

127-2.5

Sigma-Aldrich

聚D-赖氨酸&层粘连蛋白(2.5 ML)

别名:

来自人胎盘的IV型胶原蛋白

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.75

表单

liquid

质量水平

100

保质期

1 yr

技术

cell culture | mammalian: suitable

运输

wet ice

储存温度

2-8°C

一般描述

多聚-D-赖氨酸（PDL）和层粘连蛋白溶液非常适合用于细胞培养表面的涂层方案。PDL层粘连蛋白涂层广泛用于神经培养。已知它在体外细胞培养过程中可以改善细胞粘附和细胞成熟。多聚-D-赖氨酸和层粘连蛋白溶液是在磷酸盐缓冲生理盐水中进行制备并经无菌过滤。它用于涂覆组织培养器皿以达到0.25 ml/cm2的培养物表面比。组织培养器皿在室温下涂覆一小时并小心吸出溶液。在以PBS洗涤两次后，涂覆的组织培养器皿可立即使用或风干后置于4oC保存最长一周。

应用

多聚-D-赖氨酸&层粘连蛋白（2.5 ML）已用作体外诱导剂，刺激 Y79 细胞表达神经元和神经胶质细胞特征。它已用于涂布视网膜神经节细胞培养的培养板。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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