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SCR127

Sigma-Aldrich

Synthetic Laminin

Synthetic peptide, powder, suitable for the in vitro culture of rat neural stem cells

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

用于大鼠神经干细胞的合成层粘连蛋白肽,

化驗

>95% (HPLC)

品質等級

形狀

lyophilized

技術

cell culture | mammalian: suitable

輸入

sample type neural stem cell(s)

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

一般說明

层粘连蛋白是细胞基底膜的一种细胞外基质蛋白,参与多种生物学活动,包括细胞粘附,增殖,迁移,侵袭和分化。这些异三聚体大分子(由α,β和γ链组成)很难以一致的活性提取和纯化来自天然组织的数量。 尽管可以从培养的细胞或胎盘组织中分离出一些层粘连蛋白同工型,但在两种情况下,总蛋白产量往往相对较低,并且批次之间存在差异。 因此,对于许多干细胞科学家来说,有必要测试每个新的批次层粘连蛋白,以确保蛋白质的活性水平足以维持其干细胞培养。

为了克服这一时间障碍,具有与天然层粘连蛋白相似的结合和功能特性的确定的合成层粘连蛋白底物被认为是非常有利的,因为它将消除测试和筛选新批次天然蛋白的要求。 Millipore’s合成层粘连蛋白肽是天然层粘连蛋白的合适替代品,并且已被优化以在体外特异性支持大鼠神经干细胞(NSC)的细胞粘附、增殖和多谱系分化。 在涂有合成层粘连蛋白肽的组织培养板上生长的大鼠神经干细胞显示出特征性的神经干细胞标记物Nestin和Sox-2,并且还具有优先分化神经胶质和神经谱系的能力。 Millipore’s合成层粘连蛋白肽是一种廉价、可重复且确定的ECM底物替代品,可用于大鼠神经干细胞的体外培养。

(注:该产品经过优化以支持大鼠NSC的生长,不建议与人或小鼠NSC一起使用)。

包裝

5 x 3 mg

外觀

冻干肽

儲存和穩定性

合成层粘连蛋白肽(目录号SCR127)以冻干形式运输,接收后应在-20°C下储存。 一旦肽被复溶,应将其储存在4ºC下并在1个月内使用。

法律資訊

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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