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形狀
lyophilized powder
品質等級
比活性
100-300 units/mg protein
分子量
~175 kDa
成份
Protein, 65-85%
異物活動
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
儲存溫度
2-8°C
生化/生理作用
来自假单胞菌属的肌酐酶是一种每个亚基分子量为28.4 kDa的同六聚体酶。它是一种环状酰胺水解酶,可催化肌酸酐向肌酸的可逆性转化。每个单体在β-链的C末端和主要α-螺旋的N末端附近都含有一个双核锌中心。这些锌离子指示着活性位点的位置。[2]
物理性質
等电点: 4.7
米氏常数:3.2 x 10‾2M(肌苷),5.7 x 10‾2M(肌酸)
结构:每摩尔的酶6个亚基(每个亚基结合一摩尔的锌)
抑制剂:Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值: 6.5 − 7.5
最适温度: 70°C
pH稳定性: pH 7.5 − 9.0 (5°C,16小时)
热稳定性: 低于70°C(pH 7.5,30分钟)
米氏常数:3.2 x 10‾2M(肌苷),5.7 x 10‾2M(肌酸)
结构:每摩尔的酶6个亚基(每个亚基结合一摩尔的锌)
抑制剂:Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值: 6.5 − 7.5
最适温度: 70°C
pH稳定性: pH 7.5 − 9.0 (5°C,16小时)
热稳定性: 低于70°C(pH 7.5,30分钟)
單位定義
在pH 8.0、25 °C条件下,一个单位将在每分钟内水解1.0 μmole的肌苷
外觀
含有蔗糖和BSA作为稳定剂的冻干粉末
分析報告
通过缩二脲法测定的蛋白质。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Barbara Beuth et al.
Journal of molecular biology, 332(1), 287-301 (2003-08-30)
Creatinine amidohydrolase (creatininase; EC 3.5.2.10) from Pseudomonas putida, a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit, is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. The enzyme plays a key role in the
Won K Han et al.
Clinical journal of the American Society of Nephrology : CJASN, 4(5), 873-882 (2009-05-02)
Serum creatinine (Scr) does not allow for early diagnosis of acute kidney injury (AKI). The diagnostic utility of urinary kidney injury molecule-1 (KIM-1), N-acetyl-beta-D-glucosaminidase (NAG), and neutrophil gelatinase associated lipocalin (NGAL) was evaluated for the early detection of postoperative AKI
V D Luk''ianchuk et al.
Eksperimental'naia i klinicheskaia farmakologiia, 69(4), 51-56 (2006-09-26)
The influence of lipoic acid on the energy homeostasis in rats with chronic generalized parodontitis has been investigated. Lipoic acid possesses the energy-saving activity, which is related to an increase in oxidative phosphorylation efficiency.
Hariprasad Trivedi et al.
Postgraduate medicine, 121(5), 166-170 (2009-10-13)
The nephrotoxicity of gadolinium-based magnetic resonance contrast media has not been adequately studied. We evaluated the nephrotoxicity of gadolinium-based contrast media in hospitalized patients who underwent magnetic resonance imaging (MRI) as part of routine clinical care. Subjects who had a
Chronic kidney disease: Automated eGFR reporting: good for the patient?
Andrew D Rule et al.
Nature reviews. Nephrology, 5(12), 673-675 (2009-11-26)
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