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表单
lyophilized powder
比活
≥25 units/mg protein
分子量
~260 kDa
组成
Protein, ≥15% biuret
储存温度
−20°C
应用
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
- in creating creatinine-sensing membrane for biophysical studies
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
生化/生理作用
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.
物理属性
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
单位定义
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.
外形
Lyophilized powder containing mannitol as stabilizer
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
R G Washburn et al.
The Journal of antimicrobial chemotherapy, 17(5), 673-677 (1986-05-01)
Creatinine iminohydrolase (EC 3.5.4.21) quantitatively releases ammonia from flucytosine (5FC) as well as from creatinine. Using 39 sera from eight patients receiving the combination of amphotericin B with 5FC, we demonstrated that this rapid enzymatic reaction provides a valid measure
Z K He et al.
Analytical biochemistry, 283(2), 166-174 (2000-07-25)
A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream
C S Rui et al.
Annals of the New York Academy of Sciences, 672, 264-271 (1992-11-30)
A flow-injection analysis biosensor system was developed for the amperometric assay of creatinine based on coupled reactions of three immobilized enzymes, using an oxygen electrode as the detection device. The ammonia produced by creatinine deiminase-catalyzed hydrolysis of creatinine was further
Hong Dinh Duong et al.
Sensors (Basel, Switzerland), 17(11) (2017-11-09)
In this study, the oxazine 170 perchlorate (O17)-ethylcellulose (EC) membrane was successfully exploited for the fabrication of creatine- and creatinine-sensing membranes. The sensing membrane exhibited a double layer of O17-EC membrane and a layer of enzyme(s) entrapped in the EC
Manuel Gutiérrez et al.
Biosensors & bioelectronics, 23(6), 795-802 (2007-10-13)
Urea and creatinine biosensors based on urease and creatinine deiminase, respectively, covalently immobilized onto ammonium selective electrodes, were included in an array together with sensors sensitive to ammonium, potassium and sodium. Generic sensors to alkaline ions were also included. All
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