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U1500

Sigma-Aldrich

尿素酶 来源于洋刀豆 （刀豆）

Type III, powder, 15,000-50,000 units/g solid

别名:

Jack bean urease, Urea amidohydrolase

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About This Item

CAS号:
9002-13-5
EC 号:
3.5.1.5 (BRENDA, IUBMB)
EC 号:
232-656-0
MDL编号:
MFCD00070858
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

Canavalia ensiformis

类型

Type III

表单

powder

比活

15,000-50,000 units/g solid

分子量

~544620 Da

应用

diagnostic assay manufacturing

储存温度

2-8°C

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一般描述

亚基分子量：约 90,770
由六个亚基组成，总分子量：约 544620
脲酶是一种胞质酶，它会催化尿素水解为氨和二氧化碳，首先转化为氨基甲酸酯，然后氨基甲酸酯在水解时进一步分解为氨和碳酸/碳酸盐。 脲酶是含有镍辅因子的金属酶。 脲酶天然存在于植物、真菌和某些细菌中。 詹姆斯·B·萨姆纳（James B.Sumner）于1926年首次从矮刀豆中分离出了脲酶。

生化/生理作用

来自洋刀豆的脲酶通过释放由昆虫肠道中发现的蛋白酶切割的有毒小肽起杀虫剂的作用。

单位定义

一微摩尔单位，在 pH7.0，25℃ 下，每分钟从尿素中释放出 1.0 μmole 的 NH3。它相当于1.0 I.U. 或 0.054 Sumner 单位（在 pH 7.0，20°C，5 分钟内 1.0 mg 氨氮）

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000430255
0000430275
0000430275
0000430255
0000418304

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Han-Ho Kim et al.
Journal of microbiology and biotechnology, 31(12), 1684-1691 (2021-12-29)
In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment
Xiang-Zhen Yan et al.
Macromolecular bioscience, 14(7), 1004-1014 (2014-04-02)
The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be
Fernanda Stanisçuaski et al.
Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
Sun-Il Kim et al.
Microorganisms, 10(1) (2022-01-22)
Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III
Karl Norris et al.
Marine drugs, 18(6) (2020-06-06)
Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and
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