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生物源
bacterial (Actinobacillus spp.)
形狀
lyophilized powder
比活性
≥4 units/mg solid
分子量
~100 kDa
儲存溫度
−20°C
一般說明
肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成;N和C末端结构域。C端折叠在两个结构相似的结构域内,同时具有α螺旋和反平行β折叠。在这两个结构域之间,一个巯基作为活性位点,其活性无金属依赖性。
應用
肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。
生化/生理作用
肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用,并由氯化胆碱诱导。
物理性質
等电点: 4.6 ± 0.1
米氏常数: 1.9 x 10‾2M(肌酸)
结构: 每摩尔酶2个亚基
抑制剂: Cu++,Hg++,Ag+
最佳pH: 8.0
最佳温度: 40℃
pH稳定性: pH 5.5 − 9.0(25℃,16hr)
热稳定性: 低于50℃(pH 7.5,30分钟)
米氏常数: 1.9 x 10‾2M(肌酸)
结构: 每摩尔酶2个亚基
抑制剂: Cu++,Hg++,Ag+
最佳pH: 8.0
最佳温度: 40℃
pH稳定性: pH 5.5 − 9.0(25℃,16hr)
热稳定性: 低于50℃(pH 7.5,30分钟)
單位定義
在pH 7.5、37℃条件下,1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。
外觀
含有糖和EDTA 作为稳定剂的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
Bioscience, biotechnology, and biochemistry, 70(10), 2363-2370 (2006-10-13)
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