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Merck

C2409

Sigma-Aldrich

肌酸酶 来源于放线杆菌

lyophilized powder, ≥4 units/mg solid

别名:

肌酸脒基水解酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Actinobacillus spp.)

形狀

lyophilized powder

比活性

≥4 units/mg solid

分子量

~100 kDa

儲存溫度

−20°C

一般說明

肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成;N和C末端结构域。C端折叠在两个结构相似的结构域内,同时具有α螺旋和反平行β折叠。在这两个结构域之间,一个巯基作为活性位点,其活性无金属依赖性。

應用

肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。

生化/生理作用

肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用,并由氯化胆碱诱导。

物理性質

等电点: 4.6 ± 0.1
米氏常数: 1.9 x 10‾2M(肌酸)
结构: 每摩尔酶2个亚基
抑制剂: Cu++,Hg++,Ag+
最佳pH: 8.0
最佳温度: 40℃
pH稳定性: pH 5.5 − 9.0(25℃,16hr)
热稳定性: 低于50℃(pH 7.5,30分钟)

單位定義

在pH 7.5、37℃条件下,1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。

外觀

含有糖和EDTA 作为稳定剂的冻干粉

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Balasundaram Padmanabhan et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
H Crocker et al.
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
J F Bazan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
Takashi Nakamura et al.
Bioscience, biotechnology, and biochemistry, 70(10), 2363-2370 (2006-10-13)
Aspergillus nidulans possessed 16 putative amylolytic genes consisting of 7 alpha-glucosidase (agdA-F), 7 alpha-amylase (amyA-F), and 2 glucoamylase (glaA and B) genes on the genome. Among them, the agdA, agdB, agdE, agdF, amyA, amyB, amyF, and glaB genes were induced
Pinar Esra Erden et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(2), 223-239 (2006-03-16)
A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and

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