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應用
SIGMAFAST BCIP®/NBT已被用作以下底物：
- 用于免疫印迹的抗小鼠IgG偶联碱性磷酸酶
- 用于原代成骨细胞组织化学染色中碱性磷酸酶
- 骨髓基质细胞碱性磷酸酶染色
重構
将一片溶于10 ml去离子水中，得到含有BCIP/NBT的即用型缓冲溶液，pH 9.5。
法律資訊
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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实验方案
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
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