When considering your question, the initial response was that Fast Red TR is typically used for light microscopy. I consulted Conn's Biological Stains, 10th Edition from the Biological Stain Commission and found a reference for its use in fluorescence microscopy in an article by Lizard, G et al (1997). The article is titled "Detection of human papillomavirus DNA in genital lesion by enzymatic in situ hybridization with fast red and laser confocal microscopy" and was published in the Histochemical Journal (29: 544-554).
推荐产品
产品名称
SIGMAFAST™ Fast Red TR/Naphthol AS-MX 片剂, tablet, To prepare 1 mL
品質等級
形狀
tablet
溶解度
water: 1 tablet/mL, clear to hazy, colorless to faintly yellow
儲存溫度
−20°C
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應用
SIGMAFAST Fast Red TR/萘酚AS-MX片剂已用于：
开发用作免疫化学中碱性磷酸酶活性检测的沉淀底物。主要用途是免疫组织学、免疫印迹或斑点印迹。SIGMAFAST™ Fast Red TR / Naphthol AS-MX 片剂含有0.6 mM 左旋咪唑，可阻断内源性碱性磷酸酶活性。SIGMAFAST™ Fast Red TR/Naphthol AS-MX 片剂无需额外的步骤或缓冲液来制备活性底物溶液。
重構
将每片溶于1mL 去离子水中，得到即用型缓冲溶液。
法律資訊
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險聲明
危險分類
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
儲存類別代碼
8A - Combustible corrosive hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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