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E2636

Sigma-Aldrich

ExtrAvidin®–碱性磷酸酶

buffered aqueous solution

别名:

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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About This Item

MDL號碼:
MFCD00163600
分類程式碼代碼:
12352203
NACRES:
NA.46

共軛

alkaline phosphatase conjugate

品質等級

200

形狀

buffered aqueous solution

技術

dot blot: 1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
indirect ELISA: 1:60,000

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

抗生物素蛋白 (Avidin) 是一种四聚体或二聚体的生物素结合蛋白，产生于鸟类、爬行动物和两栖动物的输卵管中，并沉积在其蛋清中。由生物素的 4 个高亲和力结合位点组成。ExtrAvidin ® 由蛋清亲和素制备而成。它是亲和纯化亲和素的改良形式，具有亲和素的高比活性和链霉亲和素的低背景染色。是一种由 阿维迪链霉菌 产生的生物素结合蛋白。ExtrAvidin已与碱性磷酸酶偶联，可用于多种技术。

應用

ExtrAvidin ™-碱性磷酸酶已被用于：
  • 斑点杂交法
  • 免疫组化（福尔马林固定、石蜡包埋切片）
  • 间接 ELISA
  • 酶联免疫吸附试验 (ELISA)
  • 酶联免疫吸附点 (ELISPOT) 分析
  • 竞争性ELISA

生化／生理作用

碱性磷酸酶催化酯水解生成磷酸。 它还促进物质的跨膜运输。

外觀

含有1 mM MgCl2、1% 牛血清白蛋白和15 mM 叠氮化钠的 0.05 M Tris-HCl 缓冲液，pH 8.0中的溶液

準備報告

亲和纯化蛋白

法律資訊

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000397811
0000383784
0000383781
0000372604
0000369712

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W E Sweeney et al.
Kidney international, 56(2), 406-413 (1999-08-05)
Recessively transmitted polycystic kidney disease (PKD) in many murine models is characterized by the initial formation of proximal tubular cysts (stage 1), followed by growth and enlargement of renal collecting tubule (CT) cysts (stage 2). Previous studies have reported that
Joanne L Maki et al.
Clinical and diagnostic laboratory immunology, 10(5), 876-881 (2003-09-11)
Fish acquire protective immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection or inoculation with I. multifiliis immobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of
Alkaline Phosphatase (2103)
Jenny Meegan et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 22(6), 856-862 (2010-11-23)
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence
C E Rutten et al.
Leukemia, 22(7), 1387-1394 (2008-04-18)
Mismatching for human leukocyte antigen (HLA)-DPB1 in unrelated donor hematopoietic stem cell transplantation (URD-SCT) has been associated with a decreased risk of disease relapse, indicating that HLA-DP may represent a target for graft-versus-leukemia (GVL) reactivity in HLA class II-expressing hematological
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实验方案

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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