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B0274

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐

tablet

别名:

BCIP® 对甲苯胺盐, X-磷酸盐 对甲苯胺盐

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About This Item

经验公式（希尔记法）:
C8H6BrClNO4P · C7H9N
CAS号:
6578-06-9
分子量:
433.62
EC 号:
229-506-1
MDL编号:
MFCD00040636
UNSPSC代码:
12352204
PubChem化学物质编号:
57653906
NACRES:
NA.83

表单

tablet

储存温度

−20°C

SMILES字符串

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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相关类别

特异性

Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.

应用

用于碱性磷酸酶标记分子的比色检测。
用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（BCIP）和硝基蓝四唑（NBT）。BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、原位杂交和免疫组化。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。

数量

Contains 25 mg substrate per tablet.

底物

碱性磷酸酶的组织化学底物。

法律信息

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBP1527V
SLBH3594V
SLBG6142V
SLBG0407V
SLBF3724V

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BCIP®/NBT液体底物系统 ready to use solution

Sigma-Aldrich

B1911

BCIP®/NBT液体底物系统

Carolyn Byrne et al.
Methods in molecular biology (Clifton, N.J.), 289, 127-136 (2004-10-27)
The skin as a surface organ is uniquely accessible for whole embryo/fetal analyzes of developmental changes, such as gene induction, protein expression, formation of epidermal-derived appendages such as hair follicles, and formation of the protective barrier. Such analyses have emphasized
Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates.
J P Horwitz et al.
Journal of medicinal chemistry, 9(3), 447-447 (1966-05-01)
Michael Alifrangis et al.
Malaria journal, 3, 16-16 (2004-06-15)
The aim of this study was to develop site-specific antibodies as a tool to capture Plasmodium falciparum-dihydrofolate reductase (Pf-DHFR) from blood samples from P. falciparum infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point
Audrey Fouchs et al.
Biology of the cell, 102(8), 447-456 (2010-04-10)
Activation of MAPKs (mitogen-activated protein kinases), in particular ERK1/2 (extracellular-signal-regulated kinase 1/2), has been reported to take place in a large variety of cell types after hypo-osmotic cell swelling. Depending on cell type, ERK1/2 phosphorylation can then serve or not
M S Blake et al.
Analytical biochemistry, 136(1), 175-179 (1984-01-01)
A rapid, sensitive method has been developed to detect antibody-antigen complexes on "Western blots." The methods of H. Towbin, T. Staehlin, and J. Gordon were used to separate and blot the antigens onto nitrocellulose. The remaining sites of attachment were
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商品

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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