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Merck

72091

Sigma-Aldrich

NBT-BCIP® 溶液

BioReagent

别名:

BCIP®/NBT, NBT-X-磷酸盐

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About This Item

UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.21

产品线

BioReagent

表单

liquid

技术

microbe id | staining: suitable

溶解性

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

应用

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

SMILES字符串

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChI key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

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外形

18.8mg/ml 氯化硝基四氮唑蓝溶液;溶于 67% DMSO 的 9.4mg/ml 5-溴-4-氯-3-吲哚基磷酸酯甲苯胺盐

其他说明

在酶活性部位产生蓝色沉淀的磷酸酶显色底物

法律信息

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

152.6 °F - closed cup

闪点(°C)

67 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

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Makrina Totsika et al.
Journal of bacteriology, 191(12), 3901-3908 (2009-04-21)
Disulfide bond (DSB) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. Uropathogenic Escherichia coli (UPEC) strains possess two paralogous disulfide bond systems: the well-characterized DsbAB system
Ludovic Sauguet et al.
Nucleic acids research, 39(10), 4475-4489 (2011-02-08)
Cyclodipeptide synthases (CDPSs) belong to a newly defined family of enzymes that use aminoacyl-tRNAs (aa-tRNAs) as substrates to synthesize the two peptide bonds of various cyclodipeptides, which are the precursors of many natural products with noteworthy biological activities. Here, we
Ying Wu et al.
Infection and immunity, 76(5), 2249-2255 (2008-03-12)
The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted
Sandra A Calarota et al.
Vaccine, 26(40), 5188-5195 (2008-05-09)
IL-7 and IL-15 are key cytokines involved in the generation and maintenance of memory CD8+ T-cells. We evaluated these cytokines as molecular adjuvants for topical HIV-1 DermaVir vaccine. We found that mice receiving DermaVir formulated with HIV-1 Gag plasmid in
D Thompson et al.
BioTechniques, 12(5), 656-658 (1992-05-01)
A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels

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