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T7009

Sigma-Aldrich

Thrombin from human plasma

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

동의어(들):

Factor IIa

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크기 선택

100 UNITS
₩231,217
250 UNITS
₩415,947
1000 UNITS
₩1,364,668
5000 UNITS
₩5,244,911

₩231,217

예상 입고일2025년 4월 14일세부사항

벌크 견적 요청
모든 사진(1)

크기 선택

보기 변경
100 UNITS
₩231,217
250 UNITS
₩415,947
1000 UNITS
₩1,364,668
5000 UNITS
₩5,244,911

About This Item

CAS Number:
9002-04-4
효소 위원회 번호:
3.4.21.5 (BRENDA, IUBMB)
EC Number:
232-648-7
MDL number:
MFCD00082072
UNSPSC 코드:
12352204
NACRES:
NA.54

₩231,217

예상 입고일2025년 4월 14일세부사항

벌크 견적 요청

양식

lyophilized powder

Quality Level

200

특이 활성도

≥1,000 NIH units/mg protein (E1%/280, 18.3)

분자량

37.4 kDa

불순물

HIV and HBsAg, source material tested negative

UniProt 수납 번호

P00734

응용 분야

diagnostic assay manufacturing

저장 온도

−20°C

유전자 정보

human ... F2(2147)

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일반 설명

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.[1]

애플리케이션

Thrombin from human plasma has been:
  • dissolved in CaCl2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production[2]
  • to polymerize fibrin on well plates[3]
  • used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells[4]

생화학적/생리학적 작용

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Thrombin is an endolytic serine protease[5] which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor.[6] It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B.[5] The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer.[5] Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.[7]

단위 정의

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

물리적 형태

Lyophilized from saline sodium citrate buffer, pH 6.5

분석 메모

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

기타 정보

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

기질

제품 번호
설명
가격

저해제

제품 번호
설명
가격

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334

예방조치 성명서

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000410046
0000389789
0000371958
0000371961
0000410046

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COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Epidemiology and pathophysiology of cancer-associated thrombosis
Noble S and Pasi J
British Journal of Cancer, 102(S1), S2-S2 (2010)
Integrin alphavbetabgr3-RGDS interaction mediates fibrin-induced morphological changes of glomerular endothelial cells
Xu Q, et al.
Kidney International, 56(4), 1413-1422 (1999)
Signal transduction for proteinase-activated receptor-2-triggered prostaglandin E2 formation in human lung epithelial cells
Kawao N, et al.
Journal of Pharmacology and Experimental Therapeutics, 315(2), 576-589 (2005)
B J Biemond et al.
Circulation, 96(5), 1612-1615 (1997-10-07)
In many case-control as well as epidemiological studies, increased lipoprotein(a) [Lp(a)] levels are considered to constitute an independent risk factor for premature coronary artery and cerebrovascular disease. Lp(a) resembles an LDL particle with an additional linked protein [apolipoprotein(a), apo(a)], whose
Protease-activated receptor 3 is a second thrombin receptor in humans
Ishihara H, et al.
Nature, 386(6624), 502-502 (1997)
모든 관련된 서류 보기

문서

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

질문

1–8 / 8 질문  

  1. How much is 100UN of thrombin(T6884)? what is the weight in mg?

    1 답변

    1. Both of these Thrombin products - T7009 and T6884 - are sold by activity or units. The amount of powder in the vial is lot specific and based on the activity reported in the Certificate of Analysis.

      T6884-100UN, lot SLCP0517 tested at 2736 uits per milligram. The 100UN vial contains 36ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T6884-100UNSLCP0517.pdf

      T7009-100UN, lot SLCN9908 tested at 1742 units per milligram. The 100UN vial contains 57ug of powder per vial. See the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T7009-250UNSLCN9908.pdf

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  2. How much does the T-7009-100UN weigh with and without packaging? Country of origin Germany?

    1 답변

    1. This product is packaged in units, the exact amount of product will depend on the lot-specific activity. For example, the SLCN9908 has a specific activity of 1742 units/ mg protein. The end user will receive 0.06 mg. The net weight of packaging is 1,000 mg. The product is manufactured in the USA.

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  3. What is the solution stability of Product T7009, Thrombin from human plasma?

    1 답변

    1. By analogy to thrombin from bovine plasma (T4648), we expect stock solutions of thrombin from human plasma to be stable for one week kept refrigerated at 2-8° C. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store frozen at -20°C or colder for long term storage.

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  4. What is the isoelectric point of Product T7009, Thrombin from human plasma?

    1 답변

    1. The isoelectric point (pI) range of the human thrombin isozymes is 6.35 - 7.6. The major isoform has a pI of 7.31.

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  5. For thrombin, how do NIH units compare to International Units (IU)?

    1 답변

    1. One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

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  6. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is the solubility of Product T7009, Thrombin from human plasma?

    1 답변

    1. Thrombin from human plasma gave a clear very faint yellow solution when dissolved at 100 unit vial plus 1 ml of 28 mM sodium barbital, 0.125M sodium chloride, 1% BSA, 0.5% PEG, pH 7.35.  Thrombin from bovine plasma (T4648) is soluble in water at 10 mg/mL.

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  8. What is the cross reactivity of Thrombin?

    1 답변

    1. Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

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