T7326

Thrombin from bovine plasma

≥60 NIH units/mg protein (biuret)

• 동의어(들): Factor IIa
• CAS Number: 9002-04-4
• 효소 위원회 번호: 3.4.21.5 (BRENDA, IUBMB)
• MDL number: MFCD00166053
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: lyophilized powder
• 특이 활성도: ≥60 NIH units/mg protein (biuret)
• 구성: Protein, ≥50%
• 저장 온도: −20°C

설명

일반 설명

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin comprises of A-chain and B-chain with the catalytic pocket. The C-terminal regions has a fibrinogen recognition site (FRS) and a heparin binding site (HBS). The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and coordinates with protease-activated receptors (PARs) to regulate physiological functions. High levels of thrombin causes neurotoxicity in dopaminergic neurons and contribute to the progression of parkinsons disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.

애플리케이션

Thrombin from bovine plasma may be used:

• for the generation of fibrin from fibrinogen embedded in microbubbles
• for the cleavage of hydrogenase maturation protein (HypF-N) from the nickel resin

as revitalization model to mimic blood fabrication in root canal

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate coagulation abnormalities in acute liver failure.

생화학적/생리학적 작용

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

단위 정의

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

물리적 형태

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

제조 메모

Traditional thrombin products are activated with bovine brain thromboplastin, whereas T7326 is activated with bovine lung thromboplastin and does not contain any bovine brain products in its preparation.

분석 메모

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

기타 정보

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)