10602400001

Thrombin

from human plasma

• 동의어(들): coagulation factor Iia, thrombin
• 효소 위원회 번호: 3.4.21.5 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: human plasma
• Quality Level: 100
• 양식: lyophilized
• 특이 활성도: ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
• 분자량: M_r ~33.6 kDa
• 포장: pkg of 20 U
• 제조업체/상표: Roche
• 최적 pH: 8.2-9.0
• 배송 상태: wet ice

설명

일반 설명

Thrombin is a Na⁺ activated allosteric serine protease. It belongs to chymotrypsin family.[1] Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.[1]

Thrombin is a coagulation factor IIa.[2] It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg.[3] The enzyme converts fibrinogen to fibrin.[4] The product contains EDTA and additional stabilizing agents.

애플리케이션

Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members.[5] It has also been used in platelet spreading on fibrinogen[6][7] and immunostaining.[8]

Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.[8][9]

생화학적/생리학적 작용

Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.[1]

물리적 형태

Lyophilizate

제조 메모

Storage conditions (working solution): -15 to -25°C

Inhibitors: DFP, TLCK, PMSF, benzamidine, α₁-antitrypsin, α₂-macroglobulin, antithrombin III heparin, hirudin, and APMSF

재구성

The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!

저장 및 안정성

Store at 2 to 8 °C. (Store dry!)

기타 정보

For life science research only. Not for use in diagnostic procedures.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H332,H373
• 예방조치 성명서: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash