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P7626

Sigma-Aldrich

Phenylmethanesulfonyl fluoride

powder, ≥98.5% (GC)

동의어(들):

α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride

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크기 선택

250 MG
₩105,560
1 G
₩115,507
5 G
₩207,669
25 G
₩567,588
100 G
₩2,256,853

₩105,560

예상 입고일2025년 1월 31일세부사항

기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
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모든 사진(2)

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보기 변경
250 MG
₩105,560
1 G
₩115,507
5 G
₩207,669
25 G
₩567,588
100 G
₩2,256,853

About This Item

실험식(Hill 표기법):
C7H7FO2S
CAS Number:
329-98-6
Molecular Weight:
174.19
Beilstein:
2088311
EC Number:
206-350-2
MDL number:
MFCD00007424
UNSPSC 코드:
12352202
PubChem Substance ID:
24898863
NACRES:
NA.77

₩105,560

예상 입고일2025년 1월 31일세부사항

기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요
벌크 견적 요청

제품명

Phenylmethanesulfonyl fluoride, ≥98.5% (GC)

생물학적 소스

synthetic

Quality Level

300

분석

≥98.5% (GC)

양식

powder

mp

91-94 °C

solubility

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor, effective against such enzymes as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor via sulfonation of the hydroxyl residues of serine residues at the reactive sites of serine proteases.

애플리케이션

PMSF is often used in lysis buffer to assist in preserving proteins of interest during protein isolation and sample preparation, by inhibiting proteases that would otherwise degrade proteins after cell or tissue lysis. PMSF is unstable in aqueous media. Thus stock solutions of PMSF are generally prepared in anhydrous organic solvents (e.g. 100% ethanol, or anhydrous isopropanol) prior to use in aqueous media.

Noted general features and benefits of PMSF include the following:
  • Inhibits serine proteases such as trypsin and chymotrypsin
  • Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase
  • Not as effective or as toxic as DFP
  • Effective concentration 0.1-1 mM
  • Half-life = 1 hr. at pH 7.5
Phenylmethanesulfonyl fluoride has been used in following applications:
  • cell fractionation.[1]
  • used as a supplement in nuclear protein extraction.[1][2]
  • inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).[3]
  • used for the collection of blood prior to centrifugation to quantify plasma ANP levels.[4]

생화학적/생리학적 작용

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.[5]

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

관련 제품

제품 번호
설명
가격

픽토그램

Skull and crossbonesCorrosion
GHS06,GHS05

신호어

Danger

유해 및 위험 성명서

H301,H314

Hazard Classifications

Acute Tox. 3 Oral - Skin Corr. 1B

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCM4458
BCCL3574
BCCM0245
BCCK4462
BCCJ7880

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
Salivary esterase activity and its association with the biodegradation of dental composites.
Finer Y and Santerre JP.
Journal of Dental Research, 83(1), 22-26 (2004)
Estrogen receptors activate atrial natriuretic peptide in the rat heart.
Jankowski M, et al.
Proceedings of the National Academy of Sciences of the USA, 98(20), 11765-11770 (2001)
Lise Boussemart et al.
Nature, 513(7516), 105-109 (2014-08-01)
In BRAF(V600)-mutant tumours, most mechanisms of resistance to drugs that target the BRAF and/or MEK kinases rely on reactivation of the RAS-RAF-MEK-ERK mitogen-activated protein kinase (MAPK) signal transduction pathway, on activation of the alternative, PI(3)K-AKT-mTOR, pathway (which is ERK independent)
Rui Chen et al.
The Journal of biological chemistry, 287(36), 30800-30811 (2012-07-19)
Hypoxia-inducible factors (HIFs) are oxygen-sensitive transcription factors. HIF-1α plays a prominent role in hypoxic gene induction. HIF-2α target genes are more restricted but include erythropoietin (Epo), one of the most highly hypoxia-inducible genes in mammals. We previously reported that HIF-2α
모든 관련된 서류 보기

문서

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

프로토콜

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

질문

1–7 / 7 질문  

  1. Can this PMSF powder be dissolved in DMSO, and if yes, then to what concentration where and how to store the reconstituted vial?

    1 답변

    1. The solubility of this product in DMSO has not been tested. A 200 mM stock solution can be prepared using ethanol, methanol, and 2-propanol. It is known to have 100 mM solubility in DMSO. The product is insoluble in aqueous solutions. Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C. Storing in a desiccator may be useful.

      도움이 되었습니까?

  2. At what temperature should the stock Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?

    1 답변

    1. A 200 mM PMSF solution in dry solvent has been reported to remain active for at least 9 months at
      2-8 °C. 100 mM PMSF solutions in isopropanol may be stored at –20 °C for several months. For more information please view the Product Data Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/427/p7626pis-ms.pdf

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  3. What is the effective amount of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), to be used?

    1 답변

    1. The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.

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  4. How should stock solutions of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), be stored?

    1 답변

    1. A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.

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  5. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What does Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), inhibit?

    1 답변

    1. PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.

      도움이 되었습니까?

  7. How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?

    1 답변

    1. Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.

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