추천 제품
생물학적 소스
bovine pancreas
Quality Level
유형
Type V-S
형태
powder
특이 활성도
≥8,500 BAEE units/mg protein (biuret)
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
cow ... TRYP8(282603)
관련 카테고리
일반 설명
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
애플리케이션
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
생화학적/생리학적 작용
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
단위 정의
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
제조 메모
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
기타 정보
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
기질
제품 번호
설명
가격
억제제
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
개인 보호 장비
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Amy R Quinn et al.
The Journal of endocrinology, 214(3), 289-299 (2012-06-23)
Erratic regulation of glucose metabolism including hyperglycemia is a common condition in premature infants and is associated with increased morbidity and mortality. The objective of this study was to examine histological and ultrastructural differences in the endocrine pancreas in fetal
Shahid Mehmood et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10832-10836 (2012-06-20)
The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA
R F Regan et al.
Journal of neurochemistry, 70(1), 77-85 (1998-01-09)
The effect of magnesium on the oxidative neuronal injury induced by hemoglobin was assessed in murine cortical cell cultures. Exposure to 5 microM hemoglobin in physiologic (1 mM) magnesium for 26 h resulted in the death of about one-half the
Shin-Ichi Nakakita et al.
Methods in molecular biology (Clifton, N.J.), 2132, 567-583 (2020-04-20)
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Caroline Chupin et al.
Vaccines, 9(10) (2021-10-27)
The development of a live-attenuated vaccine (LAV) for the prevention of human metapneumovirus (HMPV) infection is often hampered by the lack of highly efficient and scalable cell-based production systems that support eventual global vaccine production. Avian cell lines cultivated in
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