A1153

Aprotinin

3-8 TIU/mg solid, lyophilized powder

• 동의어(들): BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
• 실험식(Hill 표기법): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• CAS Number: 9087-70-1
• Molecular Weight: 6511.44
• EC Number: 232-994-9
• MDL number: MFCD00130541
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid
• 생물학적 소스: bovine lung
• 형태: lyophilized powder
• 특이 활성도: 3-8 TIU/mg solid
• 분자량: ~6,500
• solubility: H₂O: ≥5 mg/mL
• UniProt 수납 번호: P00974
• 저장 온도: 2-8°C
• InChI key: ZPNFWUPYTFPOJU-UHFFFAOYSA-N
• 유전자 정보: cow ... PTI(404172)

설명

일반 설명

Aprotinin from bovine lung is a globular polypeptide monomer with a molecular weight of 6.5 kDa. Commonly used as a non-specific serine protease inhibitor, aprotinin contains an antiparallel β sheet, N-terminal 310 helix and C-terminal and α helix. Aprotinin residues from amino acids 13 - 18 are essential for binding to serine proteases.

애플리케이션

Aprotinin from bovine lung has been used:

• as a protease inhibitor in radioimmunoprecipitation assay buffer (RIPA) for the homogenization of cardiac microvascular endothelial cells (CMECs)(4) and mammary epithelial cells
• in angiogenesis assay for fibroblast
• in the proteomic stabilization of saliva supernatant

Aprotinin is largely used as an inhibitor of trypsin.

생화학적/생리학적 작용

Aprotinin inhibits proteases like trypsin, plasmin, chymotrypsin and thrombin. It blocks the bradykinin synthesis from kininogen. It is useful for treating blood loss during surgery.

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

단위 정의

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

제조 메모

This product is a lyophilized powder with activity of 3-8 TIU/mg of solid powder. Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions tend to be less stable than concentrated ones, though solution stability also depends on pH. Aprotinin is relatively stable against denaturation - only thermolysin has been found capable of degrading it at 60-80°C. Sterilizaiton of Aprotinin can be achieved through filtration by a 0.2 μm filter.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)