This product may be prepared in 10 mM Tris-HCl, pH 8.0 at 10 mg/mL. The material is also soluble in water at 10 mg/mL. Aqueous solutions are stable for at least one month when stored at 2-8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
L6876
Lysozyme from chicken egg white
powder or granules, ≥39,000 units/mg protein
동의어(들):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
로그인조직 및 계약 가격 보기
크기 선택
1 G
₩121,597
5 G
₩472,686
10 G
₩629,503
25 G
₩1,388,926
100 G
₩4,782,274
모든 사진(6)
크기 선택
보기 변경
1 G
₩121,597
5 G
₩472,686
10 G
₩629,503
25 G
₩1,388,926
100 G
₩4,782,274
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54
추천 제품
생물학적 소스
chicken egg white
양식
powder or granules
특이 활성도
≥39,000 units/mg protein
분자량
single-chain 14.3 kDa
기술
cell based assay: suitable
적합성
suitable for cell lysis
UniProt 수납 번호
응용 분야
cell analysis
저장 온도
room temp
유전자 정보
chicken ... LYZ(396218)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
애플리케이션
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
생화학적/생리학적 작용
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
특징 및 장점
Lysozyme from chicken egg white is a versatile enzyme with unique properties that make it effective for various applications, including bacterial cell wall breakdown, genomic DNA extraction, and external standard for mass analysis. It is reliable, active over a broad pH range, and available in various packaging sizes.
단위 정의
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
기질
또한 이 제품과 함께 일반적으로 구입
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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문서
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
프로토콜
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
관련 콘텐츠
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Chromatograms
application for HPLC-
I have ordered this product and stored in -70 degree C. But there is no dilution preparation method available here. Can you please tell me hot to prepare that.
1 답변-
도움이 되었습니까?
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What's the best buffer and pH for the preparation of lysozyme stock solution, and can I incubate it at room temperature for 1 day?
1 답변-
This product can be resuspended in 350 µL of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl,
1 mM EDTA, and 5% [w/v] TRITON® X-100). The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).During this assay, the end-user should Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Cat. No. T0918) with 25 µg/mL tetracycline (Cat. No. T3383) and 25 µg/mL ampicillin (Cat. No. A9518). The
optimal pH for E. coli cell lysis is 8.0 ± 0.1.16For more information please read through the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
도움이 되었습니까?
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How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?
1 답변-
For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2-8 °C.
도움이 되었습니까?
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What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?
1 답변-
Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?
1 답변-
Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.
도움이 되었습니까?
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Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?
1 답변-
The following procedure is for the lysis of E. coli. It may be used as a guideline for other species.1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518).2. Centrifuge 1-2 ml samples of the overnight culture.3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100).4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0).5. Mix by vortexing for 3 seconds.6. Incubate the lysis mixture for 30 minutes at 37 °C7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.8. Centrifuge the lysis mixture at 14,000 x g.9. Remove the pellet (cell debris) from the tube using a sterile toothpick.10. Plasmid DNA from the supernatant may then be purified and analyzed.
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