This product may be prepared in 50 mM TES buffer, pH 7.0, with 1 mM MgCl2, at 1 mg/mL. It is also soluble in water or TE buffer. Please see the link below to review the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/382/514/m9901pis-ms.pdf
M9901
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
동의어(들):
Mutanolysin
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크기 선택
1000 UNITS
₩138,243
5000 UNITS
₩273,949
10000 UNITS
₩393,414
50000 UNITS
₩1,151,010
모든 사진(2)
크기 선택
보기 변경
1000 UNITS
₩138,243
5000 UNITS
₩273,949
10000 UNITS
₩393,414
50000 UNITS
₩1,151,010
About This Item
추천 제품
생물학적 소스
bacterial (Streptomyces globisporus)
Quality Level
양식
lyophilized powder
특이 활성도
≥4000 units/mg protein (biuret)
분자량
23 kDa
적합성
suitable for cell lysis
응용 분야
diagnostic assay manufacturing
배송 상태
wet ice
저장 온도
−20°C
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일반 설명
Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. [1]
애플리케이션
Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. [2] It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. [3] It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
생화학적/생리학적 작용
Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
단위 정의
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
물리적 형태
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
법적 정보
Ficoll is a registered trademark of Cytiva
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
Matthew J Sullivan et al.
Applied and environmental microbiology, 84(18) (2018-07-15)
Group B streptococcus (GBS) is a Gram-positive bacterium associated with various diseases in humans and animals. Many studies have examined GBS physiology, virulence, and microbe-host interactions using diverse imaging approaches, including fluorescence microscopy. Strategies to label and visualize GBS using
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other
프로토콜
This procedure may be used for Mutanolysin products.
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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Hi, Could you advise the best way to resuspend mutanolysin M9901. Will it be soluble in water?
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