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Merck
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87444

Sigma-Aldrich

5-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥95.0% (HPLC)

동의어(들):

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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크기 선택

5 MG
₩371,490

₩371,490

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청
모든 사진(1)

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5 MG
₩371,490

About This Item

실험식(Hill 표기법):
C29H19NO11
CAS Number:
150206-05-6
Molecular Weight:
557.46
MDL number:
MFCD00037466
UNSPSC 코드:
12352108
PubChem Substance ID:
57653248
NACRES:
NA.32

₩371,490

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청

Grade

for fluorescence

분석

≥95.0% (HPLC)

양식

powder

solubility

DMF: soluble
DMSO: soluble

형광

λex 492 nm; λem 517 nm

적합성

suitable for fluorescence

저장 온도

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

기타 정보

Continuous determination of the intracellular pH in bacteria by fluorometry[1]; labeling human intervertebral disk cells in vitro for fluorescence microscopy[2]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCL9002
BCCH7934
BCCJ7672
BCCD0002
BCCB9167

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Juan Dubrot et al.
The Journal of experimental medicine, 211(6), 1153-1166 (2014-05-21)
Dendritic cells (DCs), and more recently lymph node stromal cells (LNSCs), have been described to tolerize self-reactive CD8(+) T cells in LNs. Although LNSCs express MHCII, it is unknown whether they can also impact CD4(+) T cell functions. We show
P Breeuwer et al.
Applied and environmental microbiology, 62(1), 178-183 (1996-01-01)
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate
H E Gruber et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 118-123 (2000-08-19)
We have assessed the utility of an intracellular fluorochrome, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), as a tracking label for human intervertebral disc cells in vitro. Although 5 microM provides adequate intracellular labeling for whole cell fluorescent microscopic identification of labeled
Jeremy A Goettel et al.
Blood, 125(25), 3886-3895 (2015-04-03)
Mice reconstituted with a human immune system provide a tractable in vivo model to assess human immune cell function. To date, reconstitution of murine strains with human hematopoietic stem cells (HSCs) from patients with monogenic immune disorders have not been
Anniek B van der Waart et al.
Blood, 124(23), 3490-3500 (2014-10-23)
Effective T-cell therapy against cancer is dependent on the formation of long-lived, stem cell-like T cells with the ability to self-renew and differentiate into potent effector cells. Here, we investigated the in vivo existence of stem cell-like antigen-specific T cells
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질문

1–3 / 3 질문  

  1. Can user the product 5-Carboxy-fluorescein diacetate N-succinimidyl ester 87444 in cells living infected with parasite?

    1 답변

    1. This application will need to be validated by the end user. This product is passively taken up by cells. Fluorescence occurs when the dye is hydrolyzed by esterases within the cell. The dye reacts covalently with amine groups present on intercellular proteins, which allows for long-term staining effects. This dye may be taken up by the parasite, as well.

      The link below may offer additional helpful information:
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/carboxyfluorescein-diacetate-succinimidyl-ester

      도움이 되었습니까?

  2. Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?

    1 답변

    1. Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized.

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  3. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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